The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells
The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells
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DOI：
10.1007/978-1-61779-185-7_8
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Inai, Tetsuichiro
中科院分区：
文献类型：
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作者：
Inai, Tetsuichiro
水、离子和小溶质的细胞旁运输是由上皮细胞、内皮细胞和间皮细胞的紧密连接（TJs）调节的。细胞旁通透性的延长升高和降低都与多种疾病有关。claudin家族是TJs的主要组成部分，在TJs中至少有24个完整的膜蛋白，通常在TJs中发现两个以上的claudin亚型。胞苷异构体的组合和混合比例决定了TJs细胞旁通透性。为了形成细胞旁通透性屏障，claudin必须在一个膜内横向相互作用（顺式相互作用），也必须在相邻细胞之间通过头对头结合相互作用（反式相互作用）。因此，检查claudin - claudin相互作用提供了对细胞旁通透性调节机制的见解。本研究采用含tj的MDCK II细胞和不含tj的HEK293细胞共培养系统来检测claudin-claudin相互作用。
The paracellular transport of water, ions, and small solutes is regulated by tight junctions (TJs) in epithelial, endothelial, and mesothelial cells. Both the prolonged increase and decrease of the paracellular permeability are involved in various diseases. Claudins, a family of at least 24 integral membrane proteins in TJs, are major components of TJs and usually more than two claudin isoforms are found in TJs. The combination and mixing ratios of claudin isoforms determine the paracellular permeability in TJs. To create the paracellular permeability barrier, claudins must interact laterally in one membrane (cis-interaction) and also must interact by head-to-head binding between adjacent cells (trans-interaction). Therefore, examination of claudin claudin interactions provides insights into the mechanism of regulation of the paracellular permeability. This study introduced coculture systems using TJ-bearing MDCK II cells and TJ-free HEK293 cells to examine claudin-claudin interactions.