Spatiotemporal expression of neurogenic locus notch homolog protein 1 in developing caudal spinal cord of fetuses with anorectal malformations from ETU-fed rats
Spatiotemporal expression of neurogenic locus notch homolog protein 1 in developing caudal spinal cord of fetuses with anorectal malformations from ETU-fed rats
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ETU喂养大鼠肛门直肠畸形胎儿尾髓发育中神经源性位点Notch同源蛋白1的时空表达
DOI：
10.1007/s10735-020-09900-w
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发表时间：
2020-08
影响因子：
3.2
通讯作者：
Weilin Wang
中科院分区：
文献类型：
--
作者：
Zhonghua Yang;Huimin Jia;Yuzuo Bai;Weilin Wang
并发症，如粪便污染、大小便失禁和便秘，是直肠肛门畸形(ARM)患者手术后的主要健康问题。在手臂患者术后并发症方面，脊髓尾端发育不良是一个日益重要的领域。然而，现有的研究还没有完全界定武器发展的机制。神经源性基因座缺口同源基因(Notch)信号通路由几个高度保守的蛋白质组成，参与脊髓发育过程。在这项研究中，研究了Notch1在使用乙基硫脲的大鼠手臂模型中在胎儿腰椎脊髓中的新出现的作用。免疫组织化学染色、Western印迹和定量逆转录实时聚合酶链式反应分析Notch1在胚胎第16、17、19、21天的时空表达。评估神经元标志物神经丝和重组信号结合蛋白-J蛋白的表达水平与Notch1表达的时间相关性。结果表明，与对照胚胎相比，Notch1在臂部胚胎腰骶段脊髓神经元中的表达降低。这些结果表明，Notch1在胚胎发育过程中的表达减少与胚胎发育过程中脊髓尾侧发育异常有关，提示Notch信号可能参与了胚胎腰骶椎的致病发育，并可能与手臂术后并发症有关。
Complications, such as fecal soiling, incontinence, and constipation, are major health issues for patients with anorectal malformations (ARMs) after surgery. Dysplasia of the caudal spinal cord is an increasingly pivotal area in the field of postoperative complications for patients with ARMs. However, the existing research has not fully defined the mechanism underlying ARMs development. The neurogenic locus notch homolog (Notch) signaling pathway comprises several highly conserved proteins that are involved in spinal cord developmental processes. In the present study, the emerging role of Notch1 in fetal lumbosacral spinal cords was investigated in a rat model of ARMs using ethylene thiourea. Immunohistochemical staining, western blot and quantitative reverse transcription real-time polymerase chain reaction were utilized to analyze spatiotemporal expression of Notch1 on embryonic days (E) 16, E17, E19, and E21. The expression levels of the neuronal marker neurofilament and recombination signal-binding protein-J protein were evaluated for temporal correlations to Notch1 expression. The results implied that Notch1 expression was reduced in lumbosacral spinal cord neurons of ARMs embryos compared to control embryos. These results showed that, in ARMs embryos decreased Notch1 expression is related to the dysplasia of the caudal spinal cord during embryogenesis, indicating that Notch signaling may participate pathogenic embryonic lumbosacral spinal development and may be associated with postoperative complications of ARMs.
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DOI：
10.3171/2009.2.spine08953l
发表时间：
2009-07
期刊：
Journal of neurosurgery. Spine
影响因子：
--
作者：
T. Smoljanović;I. Bojanić;M. Čimić
通讯作者：
T. Smoljanović;I. Bojanić;M. Čimić
影响因子：
2.4
作者：
Shi-wei Zhang;Yuzuo Bai;Dan Zhang;Tao Zhang;Shu-Cheng Zhang;Dajia Wang;Wei-lin Wang
通讯作者：
Shi-wei Zhang;Yuzuo Bai;Dan Zhang;Tao Zhang;Shu-Cheng Zhang;Dajia Wang;Wei-lin Wang
DOI：
10.1007/978-3-642-38482-0_76-2
发表时间：
2019
期刊：
Pediatric Surgery
影响因子：
--
作者：
A. Bischoff;Belinda H. Dickie;M. Levitt;A. Peña
通讯作者：
A. Bischoff;Belinda H. Dickie;M. Levitt;A. Peña
影响因子：
2.7
作者：
Fujimoto, Motoaki;Takagi, Yasushi;Tanigaki, Kenji
通讯作者：
Tanigaki, Kenji
影响因子：
3.3
作者：
Lagares ‐ Tena;Millán ‐ Paredes;Lázaro ‐ García;Navarro ‐ Luna;Delgado ‐ Rivilla;Muñoz ‐ Duyos
通讯作者：
Lagares ‐ Tena;Millán ‐ Paredes;Lázaro ‐ García;Navarro ‐ Luna;Delgado ‐ Rivilla;Muñoz ‐ Duyos