Growth of an interleukin 2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF).
Growth of an interleukin 2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF).
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粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 诱导白细胞介素 2/白细胞介素 4 依赖性 T 细胞系的生长。
DOI：
10.4049/jimmunol.138.12.4288
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发表时间：
1987
影响因子：
4.4
通讯作者：
M. Horowitz
中科院分区：
文献类型：
--
作者：
T. Kupper;P. Flood;D. Coleman;M. Horowitz
活化的辅助性T细胞系的条件培养基中的淋巴因子活性通常由“特异性”淋巴因子依赖性细胞系的增殖来定义。IL 2依赖性（和表面特异性）HT-2和CTLL细胞的各种亚系现在已显示也响应于BSF-1/IL 4而增殖。D10.G4.1是一种抗原特异性克隆的T辅助细胞，最近被证明能产生IL 4而不产生IL 2，在被抗原或有丝分裂原激活后，它能分泌两种不同的细胞因子，诱导HT-2细胞的生长。这些“T细胞生长因子”（TCGF）可以通过反相高效液相色谱法（RP-HPLC）分离。这些因子之一的TCGF活性可以被IL 4特异性抗体11B 11阻断。第二TCGF活性不受11B 11或IL 2特异性抗体的影响。这种TCGF活性可以被山羊抗粒细胞-巨噬细胞集落刺激因子（GM-CSF）的多克隆抗体中和，并且具有与重组GM-CSF相同的RP-HPLC洗脱曲线。重组GM-CSF诱导HT-2细胞增殖和长期生长，但不诱导CTLL细胞增殖和长期生长，并且这种活性可以被GM-CSF的相同抗体中和。GM-CSF最为人所知的是诱导粒细胞和巨噬细胞从骨髓来源的造血前体细胞成熟和生长的因子。GM-CSF诱导某些T细胞系生长的能力表明，该分子可能在T细胞介导的免疫应答中发挥作用，作为产生该细胞因子的淋巴组织和非淋巴组织的自分泌生长因子或旁分泌刺激物。
Lymphokine activities in conditioned medium from activated helper T cell lines are most commonly defined by the proliferation of "specific" lymphokine-dependent cell lines. Various sublines of IL 2-dependent (and ostensibly specific) HT-2 and CTLL cells have now been shown to proliferate in response to BSF-1/IL 4 as well. After activation with antigen or mitogen, D10.G4.1, an antigen-specific cloned T helper cell that has recently been shown to produce IL 4 but not IL 2, secretes two distinct cytokines that induce the growth of HT-2 cells. These "T cell growth factors" (TCGF) can be separated by reversed phase high-performance liquid chromatography (RP-HPLC). The TCGF activity of one of these factors can be blocked by 11B11, an antibody specific for IL 4. The second TCGF activity is not affected by 11B11 or by antibodies specific for IL 2. This TCGF activity can be neutralized by a goat polyclonal antibody to granulocyte-macrophage colony-stimulating factor (GM-CSF), and has a RP-HPLC elution profile identical to that of recombinant GM-CSF. Recombinant GM-CSF induces both proliferation and long-term growth of HT-2 but not CTLL cells, and this activity can be neutralized by the same antibody to GM-CSF. GM-CSF is best known as a factor that induces the maturation and growth of granulocytes and macrophages from bone marrow-derived hematopoietic precursor cells. The ability of GM-CSF to induce the growth of certain T cell lines indicates that this molecule may play a role in T cell-mediated immune responses, either as an autocrine growth factor or a paracrine stimulus from both lymphoid and nonlymphoid tissues that produce this cytokine.