MESODERMAL EXPRESSION OF THE CHICKEN ERG GENE ASSOCIATED WITH PRECARTILAGINOUS CONDENSATION AND CARTILAGE DIFFERENTIATION
MESODERMAL EXPRESSION OF THE CHICKEN ERG GENE ASSOCIATED WITH PRECARTILAGINOUS CONDENSATION AND CARTILAGE DIFFERENTIATION
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DOI：
10.1016/0925-4773(94)00322-e
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发表时间：
1995-03-01
影响因子：
2.6
通讯作者：
DUTERQUECOQUILLAUD, M
中科院分区：
文献类型：
--
作者：
DHORDAIN, P;DEWITTE, F;DUTERQUECOQUILLAUD, M
ets基因超家族编码一类转录因子，其通过85个氨基酸的ETS结构域与富含嘌呤的序列结合。其中，人类erg基因已被发现与尤文氏肉瘤、儿童原始神经直肠肿瘤和急性髓性白血病有关。然而，人类的ERG表达知之甚少。北方印迹分析表明，人类erg的表达仅限于少数细胞系和胸腺，但在发育过程中的表达状态仍然未知，可能是因为尚未分离出该基因的同源物，并在其他脊椎动物中进行了研究。因此，我们选择克隆鸡erg基因（ck-erg）并研究其在鸡发育过程中的表达。我们获得了一个真正的克隆ck-erg，并确定其产物的转录调节特性。ck-Erg蛋白通过常规的共有ETS结合位点作为转录激活因子。对各种鸡组织的北方印迹研究、原位分析和与充分表征的c-ets-1表达的比较表明，ck-erg在中胚层中表达，并且在较小程度上在外胚层衍生的组织中表达。在鸡的发育过程中，可以观察到两个显著特征。从F1到E3.5，ck-erg表达广泛分布于中胚层衍生物和神经嵴，类似于c-ets-1表达。然而，E6，ck-erg的表达表现出，不像c-ets-1，一个全新的和强烈的信号在前软骨冷凝区和软骨骨骼原基。这些阶段是骨骼加工过程中骨形成的第一步。我们的研究结果表明，第一次可能的具体参与ck-erg软骨形态发生。
The ets gene superfamily encodes a class of transcription factors that bind to a purine rich sequence through a 85 amino-acid ETS domain. Among them, the human erg gene has been found to be involved in Ewing's sarcoma, primitive neurectodermal tumour of childhood and acute myeloid leukaemia. Nevertheless, little is known about human erg expression. Northern blot analyses have shown a human erg expression restricted to few cell lines and thymus, but the status concerning expression during development remains unknown probably because no homologue of this gene has yet been isolated and studied in other vertebrates. We thus choose to clone the chicken erg gene (ck-erg) and to study its expression during chicken development. We obtained a bona fide clone of ck-erg and defined the transcriptional modulating properties of its product. The ck-Erg protein acts as a transcriptional activator through a conventional consensus ETS binding site. Northern blot studies on various chicken tissues, in situ analyses and comparison with the well-characterised c-ets-1 expression show that ck-erg is expressed in mesoderm- and, to a lesser extent, in ectoderm-derived tissues. During chicken development, two salient features could be observed. From stage Fl to E3.5, ck-erg expression was widely distributed in mesodermal derivatives and neural crest, resembling c-ets-1 expression. However, by E6, the expression of ck-erg exhibited, unlike c-ets-1, a drastically new and strong signal in precartilaginous condensation zones and cartilaginous skeletal primordia. These stages are the first steps of bone formation during skeleton elaboration. Our results show for the first time a possible specific involvement of ck-erg in cartilage morphogenesis.