Effects of retinoid deficiency on the development of the heart and vascular system of the quail embryo
Effects of retinoid deficiency on the development of the heart and vascular system of the quail embryo
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类维生素A缺乏对鹌鹑胚胎心脏和血管系统发育的影响
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
M. Sporn
中科院分区：
文献类型：
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作者：
U. Heine;A. Roberts;E. Munoz;N. Roche;M. Sporn
通过光镜和扫描电镜研究了类维生素a缺乏和对照的鹌鹑胚胎（5-15 somite期）以及2和2.5 d龄胚胎，在体外和体内研究了类维生素a在生长和分化中的调节作用。根据Thompson等人1969年的描述，从缺乏类维甲酸和类胡萝卜素的饮食中饲养的鹌鹑群中获得了缺乏类维甲酸的可育蛋。如前所述，胚胎发育过程中的类维生素a剥夺会导致上皮和间充质起源器官的异常，最显著地阻止了禽类胚胎中胚胎外循环系统的形成。我们的体内研究表明，后一种缺陷的基础是原始心脏管不能在其后端打开，从而阻止了正常连接胚胎和胚胎外循环系统的脐肠系膜静脉的形成。类维生素a缺乏的早期表现也可能导致贲门裂的形成，晚期表现为单个心室扩张的发展。相反，血岛胚外血管系统发育良好。在缺陷胚胎中，由心跳速率所显示的心脏功能会降低。我们的体外研究表明，通过在缺乏维甲酸的琼脂培养基上培养24小时的正常胚胎，循环系统的发育也存在类似缺陷；相反，在含维甲酸的琼脂培养基上，观察到缺乏维甲酸的胚胎的正常发育。
SummaryThe regulatory role of retinoids in growth and differentiation has been examined in vitro and in vivo by light and scanning electron microscopy using retinoid-deficient and control quail embryos between the 5–15 somite stage, as well as 2- and 2.5-day-old embryos. Fertile, retinoid-deficient eggs were obtained from flocks of quail maintained on a retinoid- and carotenoid-deficient diet, supplemented only with small amounts of retinoic acid methyl ester as described by Thompson et al. 1969. As described previously, retinoid deprivation during embryonal development causes abnormalities in organs of epithelial and mesenchymal origin, most dramatically preventing the formation of the extraembryonal circulatory system in the avian embryo. Our in vivo studies show that the basis for the latter defect is the failure of the primitive heart tubes to open at their posterior end, thus preventing the formation of omphalomesenteric veins normally connecting the embryonal with the extraembryonal circulatory system. Early manifestation of the retinoid-deficient defect may result also in formation of a cardia bifida, late manifestation in development of a single dilated ventricle. In contrast, the extraembryonal vascular system of blood islands is well developed. Heart function as shown by the rate of heart beat is reduced in deficient embryos. Our in vitro studies demonstrate similar defects in the development of the circulatory system by culture of normal 24-h embryos on retinoid-deficient agar medium; conversely, normal development is observed upon culture of retinoid-deficient embryos on retinoid-containing agar medium.