The location and characteristics of two populations of dental pulp cells affect tooth development.
The location and characteristics of two populations of dental pulp cells affect tooth development.
复制标题
DOI：
10.1111/j.1600-0722.2008.00603.x
复制
发表时间：
2009-04
影响因子：
1.9
通讯作者：
Y. Sumita;S. Tsuchiya;I. Asahina;H. Kagami;M. Honda
中科院分区：
文献类型：
--
作者：
Y. Sumita;S. Tsuchiya;I. Asahina;H. Kagami;M. Honda
本研究探讨了猪牙冠形成初期两种牙髓细胞群的特征。一种移植方法的发展，以重现上皮细胞间充质细胞相互作用在牙发生（牙齿发育）。该技术允许两种类型的细胞/组织在体内结合。定位于颈袢上皮区域、牙髓角和牙髓核室的细胞群被分离并分离成单个细胞。通过半定量和定量逆转录聚合酶链反应（RT-PCR）分析检测每个群体的基因表达模式，并通过将颈袢上皮细胞与髓角细胞或髓核细胞结合在可生物降解的胶原支架上检测其组织形成能力，随后使用组织学和免疫组织学检查。基因表达谱显示，牙髓角细胞比牙髓核细胞成熟。颈环上皮细胞与牙髓角细胞结合主要重建牙本质-牙骨质结构。相比之下，颈袢上皮细胞与髓核细胞结合重建了牙釉质-牙本质结构。这些结果表明，当与上皮细胞结合时，位于牙髓特定位置的间充质细胞具有特定的组织形成潜力。
This study investigated the characteristics of two dental pulp cell populations during the early stages of crown formation in porcine teeth. A transplantation method was developed to reproduce epithelial cell-mesenchymal cell interactions during odontogenesis (tooth development). The technique allowed two types of cells/tissue to be combined in vivo. Populations of cells localized in the cervical loop epithelium region, dental pulp horn, and dental pulp core chambers were isolated and dissociated into single cells. Each population was examined for its gene-expression pattern using both semiquantitative and quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses, and for its tissue-formation capability by combining the cervical loop epithelial cells with either pulp horn cells or pulp core cells on biodegradable collagen scaffolds that were subsequently examined using histology and immunohistology. Gene-expression patterns showed that pulp horn cells were more mature than pulp core cells. Cervical loop epithelial cells combined with pulp horn cells mainly reconstituted dentin-cementum structures. By contrast, cervical loop epithelial cells combined with pulp core cells reconstituted enamel-dentin structures. These results suggest that mesenchymal cells residing in a specific location of the pulp possess a specific tissue-formation potential when combined with epithelial cells.