High-resolution physical mapping by combined Alu-hybridization/PCR screening: construction of a yeast artificial chromosome map covering 31 centimorgans in 3p21-p14.
High-resolution physical mapping by combined Alu-hybridization/PCR screening: construction of a yeast artificial chromosome map covering 31 centimorgans in 3p21-p14.
复制标题
通过组合 Alu 杂交/PCR 筛选进行高分辨率物理作图：构建覆盖 3p21-p14 中 31 厘摩的酵母人工染色体图谱。
DOI：
10.1073/pnas.93.9.4474
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发表时间：
1996
影响因子：
11.1
通讯作者：
Housman,DE
中科院分区：
文献类型：
--
作者：
Aburatani,H;StantonJr,VP;Housman,DE
我们描述了一种大规模物理作图的综合方法，该方法使用了一种基于PCR的直接筛选结合PCR杂交筛选策略，以构建一个连续的酵母人工染色体图谱，该图谱覆盖了人类3号染色体上>20 mb的p14-p21条带，由205个基因座组成，由480条酵母人工染色体连接，平均基因座间距离约等于100 kb。我们观察到一个反向分布的RT-PCR和（CA）n标记。这些结果表明，这两种筛选方法可能是互补的，并证明了实用程序的RT-PCR杂交筛选在高分辨率的人体物理地图的封闭。
We describe an integrated approach to large-scale physical mapping using an Alu-PCR hybridization screening strategy in conjunction with direct PCR-based screening to construct a continuous yeast artificial chromosome map covering >20 mb in human chromosome 3, bands p14-p21, composed of 205 loci, connected by 480 yeast artificial chromosomes, with average interlocus distance of approximately equal to 100 kb. We observe an inverse distribution of Alu-PCR and (CA)n markers. These results suggest that the two screening methods may be complementary and demonstrate the utility of Alu-PCR hybridization screening in the closure of high-resolution human physical maps.