The mammary gland "Side Population": A putative stem/progenitor cell marker?
The mammary gland "Side Population": A putative stem/progenitor cell marker?
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DOI：
10.1007/s10911-005-2539-0
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发表时间：
2005-01-01
影响因子：
2.5
通讯作者：
Clarke, RB
中科院分区：
文献类型：
--
作者：
Smalley, MJ;Clarke, RB
造血干细胞通过其泵出Hoechst 33342染料并形成可通过流式细胞术定义的独特群体-侧群（SP）的能力而被分离。膜泵Bcrp已被确定为SP表型的分子决定因素。具有Bcrp活性的SP群体已经在许多组织中被定义，包括小鼠乳腺和人乳腺上皮，并且已经提出SP表型是通用的干细胞标志物。对小鼠和人乳腺SP的研究表明，该群体是未分化的，但能够在体外和体内分化成管腔和肌上皮谱系的上皮结构。然而，SP富含干细胞的证据目前只是相关的，并且存在潜在的混淆技术问题。我们仍在等待SP含有干细胞群的正式证据。
Hematopoietic Stem Cells have been isolated by their ability to pump out Hoechst 33342 dye and form a distinct population definable by flow cytometry-the Side Population (SP). The membrane pump Bcrp has been identified as the molecular determinant of the SP phenotype. An SP population with Bcrp activity has been defined in a number of tissues, including mouse mammary and human breast epithelium, and it has been proposed that the SP phenotype is a universal stem cell marker. Studies of mouse and human breast SP suggest that the population is undifferentiated but capable of differentiating into epithelial structures of both luminal and myoepithelial lineages both in vitro and in vivo. However, evidence that the SP is enriched for stem cells is, at the moment, only correlative, and there are potentially confounding technical issues. We still await formal proof that the SP contains a stem cell population.