Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon
Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon
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DOI：
10.1007/s10577-010-9119-x
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发表时间：
2010-03
影响因子：
2.6
通讯作者：
Ahmet L. Tek;Kazunari Kashihara;M. Murata;K. Nagaki
中科院分区：
文献类型：
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作者：
Ahmet L. Tek;Kazunari Kashihara;M. Murata;K. Nagaki
着丝粒作为动粒的组装位点，在细胞分裂过程中对遗传物质的分配起着重要作用。为了确定大豆的功能性着丝粒，我们对大豆着丝粒特异性组蛋白H3（GmCENH 3）进行了表征，并开发了针对其N-末端的抗体。使用这种抗体，我们克隆着丝粒相关的DNA序列的染色质免疫沉淀。我们的分析表明，大豆着丝粒是由两个不同的卫星重复（GmCent-1和GmCent-4）和反转录转座子相关序列（GmCR）。大豆基因组可能的异源多倍体起源的着丝粒卫星序列进行了讨论。
The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.