The development of new biomarkers of spermatozoa quality in cattle.
The development of new biomarkers of spermatozoa quality in cattle.
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DOI：
10.3389/fvets.2023.1258295
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发表时间：
2023
影响因子：
3.2
通讯作者：
Ortega MS
中科院分区：
文献类型：
--
作者：
Fallon L;Diaz-Miranda E;Hamilton L;Sutovsky P;Zigo M;Spencer TE;Ortega MS
目前需要新的精子质量生物标志物，其一致且正确地识别将成功地有助于随后胚胎发育的精子。这可以提高精液分析的标准化，降低早期胚胎死亡率，并在为女性服务之前使用这些生物标志物作为选择工具。本研究利用成像技术来鉴定精子质量的潜在生物标志物，使用先前分类为在体外产生囊胚时表现高（n = 4）或低（n = 4）的公畜。在梯度纯化方案之前和之后对精子进行评估，以了解细胞群如何受到此类方案的影响，以及体内和体外使用之间的差异。来自低性能公畜的梯度前样品具有增加的DNA损伤发生率，尽管来自高性能公畜的梯度后样品被发现具有增加的DNA损伤发生率。当通过荧光显微镜评价形态学时，来自高性能公牛的预梯度样品中最普遍的缺陷是尾部缺陷，其在纯化处理期间被成功去除。来自低性能公畜的预梯度样品中最普遍的缺陷是位于头部的侵略性缺陷，这将在受精时带入卵母细胞并可能损害胚胎发育。采用基于图像的流式细胞术（IBFC）来量化缺陷患病率，以评价更大的样本量，从而降低手动评估中存在的变异性。使用IBFC，侵略缺陷再次确定在精子的头部从低性能的公牛。来自低性能公畜的梯度后样品比来自高性能公畜的梯度后样品具有显著更大（p < 0.05）的侵略基因缺陷发生率。此外，IBFC用于评价梯度纯化后的精子活力。确定了不同的精子细胞群。高性能的公牛有更多的精子在人口中被认为是最可行的比低性能的公牛。这项研究表明，精子缺陷在梯度纯化前后的人群中各不相同，这表明单独评估精液在体内和体外使用可能是有益的。此外，在低性能的公畜，可以解释成功受精和胚胎发育之间的差异的普遍缺陷被确定。因此，阐明了由父系调节的功能障碍，这可能会影响早期胚胎发育。
There is a current need for new biomarkers of spermatozoa quality, that consistently and correctly identify spermatozoa that will successfully contribute to subsequent embryo development. This could improve the standardization of semen analysis, decrease early embryo mortality, and use these biomarkers as a selection tool before servicing females. This study utilized imaging techniques to identify potential biomarkers of sperm quality, using sires previously classified as high (n = 4) or low (n = 4) performing at producing blastocysts in vitro. Spermatozoa were assessed before and following a gradient purification protocol, to understand how populations of cells are impacted by such protocols and may differ between in vivo and in vitro use. Pre-gradient samples from low-performing sires had an increased incidence of DNA damage, although post-gradient samples from high-performing sires were found to have an increased incidence of DNA damage. When evaluating morphology via fluorescent microscopy, the most prevalent defects in pre-gradient samples from high-performing sires were tail defects, which are successfully removed during purification processing. The most prevalent defects in pre-gradient samples from low-performing sires were aggresome defects located in the head, which would be brought into an oocyte upon fertilization and may impair embryo development. Image-based flow cytometry (IBFC) was employed to quantify defect prevalence to evaluate a greater sample size decreasing the variability that exists in manual assessments. Using IBFC, aggresome defects were again identified in the heads of spermatozoa from low-performing sires. Post-gradient samples from low-performing sires had a significantly greater (p < 0.05) incidence of aggresome defects than post-gradient samples from high-performing sires. Additionally, IBFC was used to evaluate spermatozoa viability following gradient purification. Distinct populations of sperm cells were identified. High-performing sires had more spermatozoa in the population deemed most viable than low-performing sires. This study demonstrated that spermatozoa defects vary in populations before and following gradient purification, indicating that it may be beneficial to separately evaluate semen for in vivo and in vitro use. Furthermore, a prevalent defect in low-performing sires that could explain a discrepancy between successful fertilization and embryo development was identified. Therefore, elucidating a malfunction regulated by sire, that could potentially affect early embryo development.
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影响因子：
1.7
作者：
Morrell, J. M.;Johannisson, A.;Rodriguez-Martinez, H.
通讯作者：
Rodriguez-Martinez, H.
影响因子：
1.7
作者：
Attia, S.;Katila, T.;Andersson, M.
通讯作者：
Andersson, M.
影响因子：
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作者：
Cornwall, Gail A.;von Horsten, H. Henning;Whelly, Sandra
通讯作者：
Whelly, Sandra
影响因子：
3.6
作者：
Harstine, B. R.;Utt, M. D.;DeJarnette, J. M.
通讯作者：
DeJarnette, J. M.
影响因子：
16.6
作者：
Kerns K;Zigo M;Drobnis EZ;Sutovsky M;Sutovsky P
通讯作者：
Sutovsky P