Design and Performance of an Infrared Microscope Attachment
Design and Performance of an Infrared Microscope Attachment
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红外显微镜附件的设计和性能
DOI：
10.1364/josa.43.000984
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发表时间：
1953
影响因子：
--
通讯作者：
E. H. Siegler
中科院分区：
文献类型：
--
作者：
V. Coates;A. Offner;E. H. Siegler
设计并制造了一台采用反射光学的红外显微镜，作为珀金-埃尔默单光束光谱仪的附件。它安装在分散光束中单色仪的出口狭缝之后，以最大限度地减少样品中可能产生的加热和光化学影响。增加显微镜不会破坏分光计的正常宏观功能。提供了方便的宏微观转换。聚光器和物镜对属于Schwarzschild类型，以0.75的数值孔径和0.4的遮盖率工作，并被设计为在光谱的红外区域提供最佳图像。聚光器在缩小8.5×的样品空间处形成出射狭缝的图像，并且物镜在可调节的光圈处提供样品的25×放大的图像。提供了一种观察和操纵系统，以允许小样本的准确定位。最大场量为0.650×0.220 mm。通过最大限度地减少反射面的数量和利用场镜来提供有效的能量传递，来自单色仪的大约35%的可用辐射是保守的。辐射被带到一个独立的探测器和前置放大器，它们连接到光谱仪的放大器和记录器。可以有效研究的最小样本量取决于波长，主要受可用能量的限制。讨论了最小样本量和光谱稀释度，利用该仪器获得了纤维、晶体和生物组织切片的红外吸收光谱，以及极小体积的细胞溶液。给出了绩效数据，并确定了一个优秀系数。
An infrared microscope employing reflecting optics has been designed and constructed as an attachment to Perkin-Elmer single beam spectrometers. It is mounted after the exit slit of the monochromator in the dispersed beam to minimize possible heating and photochemical effects in the sample. The normal macro functions of the spectrometer are not disrupted by the addition of the microscope. Convenient macro-micro conversion is provided.The condenser and objective pair, which are of the Schwarzschild type, operate at a numerical aperture of 0.75 with a 0.4 obscuration ratio and are designed to provide optimum imagery in the infrared region of the spectrum. The condenser forms an image of the exit slit at the sample space reduced 8.5×, and the objective provides a 25× enlarged image of the sample at an adjustable diaphragm. A viewing and manipulating system is provided to allow accurate positioning of small samples. The maximum field size is 0.650×0.220 mm. About 35 percent of the radiation available from the monochromator is conserved by minimizing the number of reflecting surfaces and by utilizing field mirrors to provide efficient energy transfer. The radiation is brought to a separate detector and preamplifier which are connected to the amplifier and recorder of the spectrometer.The minimum sample size which can be effectively studied depends on wavelength and is primarily limited by the available energy. Minimum sample size and spectral dilution are discussed.The infrared absorption spectra of fibers, crystals, and biological tissue sections, as well as solutions in cells, of extremely small volume, have been obtained using this instrument. Performance data are given and a figure of merit determined.