Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain.
Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain.
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DOI：
10.1186/s12951-016-0158-0
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发表时间：
2016-01-16
影响因子：
10.2
通讯作者：
Yu B
中科院分区：
文献类型：
--
作者：
Hou J;Liu F;Wu N;Ju J;Yu B
氯酚是一种环境污染物，具有致癌性、诱变性和细胞毒性，对生物具有很高的毒性。与传统的物理化学方法相比，细菌降解被认为是一种经济有效且环保的去除氯酚的方法。在这项研究中，我们首先开发了一种利用磁固定化红球菌细胞降解氯酚的高效工艺。R. rhodochrous DSM6263在邻位氯酚环羟基化后降解氯酚。用9 g/L Fe3O4纳米颗粒固定k-卡拉胶后，细胞对2-氯酚、4-氯酚、2,3-二氯酚及其混合物的降解效率高于游离细胞。磁性纳米颗粒固定的细胞至少可以使用6个周期。该研究为改善废水中氯酚的生物修复过程中使用的生物催化剂提供了一种有前途的技术。
Chlorophenols are environmental contaminants, which are highly toxic to living beings due to their carcinogenic, mutagenic and cytotoxic properties. Bacterial degradation has been considered a cost-effective and eco-friendly method of removing chlorophenols, compared to the traditional physical–chemical processes. In this study, we first developed an efficient process for the biodegradation of chlorophenols by magnetically immobilized Rhodococcus rhodochrous cells. R. rhodochrous DSM6263 degrades chlorophenols following the first step of hydroxylation at the ortho-positions of chlorophenolic rings. The cells immobilized by k-carrageenan with 9 g/L Fe3O4 nanoparticles could efficiently degrade 2-chlorophenol, 4-chlorophenol, 2,3-dichlorophenol and their mixture, which were even higher than those by free cells. The magnetically nanoparticle-immobilized cells could be used at least for six cycles. Given the much easier separation by an external magnetic field and high degradation efficiency, this study provides a promising technique for improving biocatalysts used in the bioremediation process for chlorophenols in wastewater.