Difference in Binocularity and Ocular Dominance Plasticity between GABAergic and Excitatory Cortical Neurons
Difference in Binocularity and Ocular Dominance Plasticity between GABAergic and Excitatory Cortical Neurons
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DOI：
10.1523/jneurosci.5025-09.2010
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发表时间：
2010-01-27
影响因子：
5.3
通讯作者：
Tsumoto, Tadaharu
中科院分区：
文献类型：
--
作者：
Kameyama, Katsuro;Sohya, Kazuhiro;Tsumoto, Tadaharu
大脑皮层中的神经元回路主要由谷氨酸能/兴奋性和GABA能/抑制性神经元组成。在出生后发育的关键时期，单眼视觉剥夺改变了视皮层神经元的双眼反应性。虽然GABA能神经元被认为在关键期的表达中起关键作用，但其双眼反应性和眼优势可塑性是否与兴奋性神经元不同尚不清楚。近年来发现，关键期的结束并不严格，因此成年人的皮层神经元仍具有一定的眼优势可塑性。然而，目前尚不清楚哪种类型的神经元或两者在成年后保持这种可塑性。为了解决这些问题，我们应用体内双光子功能性钙离子成像转基因小鼠的GABA能神经元表达一种黄色荧光蛋白称为金星。我们发现，在正常视皮层中，GABA能神经元比兴奋性神经元更具有双眼性，且在关键期内，两类神经元对单眼视觉剥夺表现出相同程度的可修饰性，但在关键期结束后，GABA能神经元的可修饰性强于兴奋性神经元。
Neuronal circuits in the cerebral cortex consist mainly of glutamatergic/excitatory and GABAergic/inhibitory neurons. In the visual cortex, the binocular responsiveness of neurons is modified by monocular visual deprivation during the critical period of postnatal development. Although GABAergic neurons are considered to play a key role in the expression of the critical period, it is not known whether their binocular responsiveness and ocular dominance plasticity are different from those of excitatory neurons. Recently, the end of the critical period was found to be not strict so that cortical neurons in the adult still have some ocular dominance plasticity. It is not known, however, which type of neurons or both maintain such plasticity in adulthood. To address these issues, we applied in vivo two-photon functional Ca2+ imaging to transgenic mice whose GABAergic neurons express a yellow fluorescent protein called Venus. We found that GABAergic neurons are more binocular than excitatory neurons in the normal visual cortex, and both types of neurons show the same degree of modifiability to monocular visual deprivation during the critical period, but the modifiability of GABAergic neurons is stronger than that of excitatory neurons after the end of the critical period.