Recurrent Respiratory Syncytial Virus Infections in Allergen-Sensitized Mice Lead to Persistent Airway Inflammation and Hyperresponsiveness1
Recurrent Respiratory Syncytial Virus Infections in Allergen-Sensitized Mice Lead to Persistent Airway Inflammation and Hyperresponsiveness1
复制标题
过敏原敏感小鼠反复呼吸道合胞病毒感染导致持续性气道炎症和高反应性1
作者：
H. Matsuse;A. Behera;Mukesh Kumar;H. Rabb;R. Lockey;S. Mohapatra
呼吸道合胞病毒（RSV）感染被认为是支气管哮喘的危险因素；然而，过敏原致敏和 RSV 感染在肺部炎症和哮喘发展中的协同作用一直存在争议。在这项研究中，在一组对照小鼠、RSV 感染小鼠、粉尘螨 (Df) 过敏原致敏小鼠、以及 Df 过敏原致敏加 RSV 感染的 BALB/c 小鼠中检查了原发性和复发性 RSV 感染对过敏性哮喘的影响。 Df 致敏小鼠的原发性 RSV 感染会暂时增加气道反应性，并伴有肺组织中嗜酸性粒细胞浸润、ICAM-1 和巨噬细胞炎症蛋白-1α (MIP-1α) 表达的增加。继发性 RSV 感染持续增强 Df 致敏小鼠的气道反应性，同时肺组织中 MIP-1α 和 RSV Ag 负荷增加。胸部淋巴结单核细胞的大量培养表明，急性 RSV 感染会增强 Th1 和 Th2 样细胞因子，而二级和三级感染则使细胞因子谱向有利于 Df 致敏小鼠中 Th2 样细胞因子反应的方向转变。仅在 RSV 再次感染后，Df 致敏小鼠的血清总 IgE 水平仍持续升高。因此，Df 致敏小鼠中反复感染 RSV 会增加 Th2 样细胞因子、总血清 IgE Ab 和 MIP-1α 的合成，这些细胞因子导致持续性气道炎症和高反应性，而这两者都是哮喘的特征。
Respiratory syncytial virus (RSV) infection is considered a risk factor for bronchial asthma; however, the synergy between allergen sensitization and RSV infection in the development of pulmonary inflammation and asthma has been controversial. In this study the effects of primary and recurrent RSV infection on allergic asthma were examined in a group of control, RSV-infected, Dermatophagoides farinae (Df) allergen-sensitized, and Df allergen-sensitized plus RSV-infected BALB/c mice. Primary RSV infection in Df-sensitized mice transiently increases airway responsiveness, which is accompanied by increases in eosinophilic infiltration, the expression of ICAM-1, and macrophage inflammatory protein-1α (MIP-1α) in the lung tissue. A secondary RSV infection persistently enhances airway responsiveness in Df-sensitized mice, with a concomitant increase in MIP-1α and RSV Ag load in lung tissues. Bulk cultures of thoracic lymph node mononuclear cells demonstrate that acute RSV infection augments both Th1- and Th2-like cytokines, whereas secondary and tertiary infections shift the cytokine profile in favor of the Th2-like cytokine response in Df-sensitized mice. The elevated total serum IgE level in the Df-sensitized mice persists following only RSV reinfection. Thus, recurrent RSV infections in Df-sensitized mice augment the synthesis of Th2-like cytokines, total serum IgE Abs, and MIP-1α, which are responsible for persistent airway inflammation and hyperresponsiveness, both of which are characteristics of asthma.
登录
查看更多内容
影响因子：
15.9
作者：
Schwarze, J;Hamelmann, E;Gelfand, EW
通讯作者：
Gelfand, EW
影响因子：
15.9
作者：
GRAHAM, BS;BUNTON, LA;KARZON, DT
通讯作者：
KARZON, DT
影响因子：
4.4
作者：
L. Cohn;J. Tepper;K. Bottomly
通讯作者：
L. Cohn;J. Tepper;K. Bottomly
鼻病毒通过单核细胞依赖性机制产生淋巴细胞非特异性激活。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gern,JE;Vrtis,R;Kelly,EA;Dick,EC;Busse,WW
通讯作者：
Busse,WW
麻疹病毒感染与 IL-4 在 IgE 类别转换中发挥协同作用。
DOI：
--
发表时间：
1999
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Imani,F;Proud,D;Griffin,DE
通讯作者：
Griffin,DE