Study of flow rate in pressurized gradient capillary electrochromatography using splitter and separation of peptides using an Amide stationary phase.
Study of flow rate in pressurized gradient capillary electrochromatography using splitter and separation of peptides using an Amide stationary phase.
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DOI：
10.1016/j.chroma.2004.03.081
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发表时间：
2004-07
期刊：
影响因子：
--
通讯作者：
Rie Nakashima;S. Kitagawa;Tatsunari Yoshida;T. Tsuda
中科院分区：
文献类型：
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作者：
Rie Nakashima;S. Kitagawa;Tatsunari Yoshida;T. Tsuda
构建了使用分流器的加压梯度毛细管电色谱仪。研究了梯度洗脱过程中流速的变化，并演示了使用酰胺固定相进行肽的分离。流速是控制色谱行为的重要因素之一，在梯度洗脱过程中流速增加，并且柱与阻力管之间流动相的不匹配导致了流速的三种变化模式。电色谱中的电泳迁移可以增强肽的分离。通过施加+5kV，牛血清白蛋白胰蛋白酶消化物的分离峰数从30增加到40。
A pressurized gradient capillary electrochromatograph using a splitter was constructed. The variation in flow rate during gradient elution was investigated and separations of peptides using an Amide stationary phase were demonstrated. The flow rate, which is one of the important factors to control chromatographic behavior, was increased during the gradient elution, and the mismatching of mobile phase between the column and the resistance tubing derived three variation patterns in the flow rate. The electrophoretic migration in electrochromatography could enhance in separation of peptides. The separated peak number of tryptic digest of bovine serum albumin was increased from 30 to 40 by the application of +5kV.