Identifications of metabolic differences between Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata Cum Melle for spleen-qi deficiency rats: A comparative study.
Identifications of metabolic differences between Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata Cum Melle for spleen-qi deficiency rats: A comparative study.
复制标题
DOI：
10.1016/j.jpba.2023.115689
复制
发表时间：
2023-08
影响因子：
3.4
通讯作者：
Yuefeng Li;Yugui Zhang;Rui Cao;Jiangtao Niu;Tian-Tian Bian-Tian;Dingcai Ma;Zhe Wang;Maomao Wang;Xingke Yan
中科院分区：
文献类型：
--
作者：
Yuefeng Li;Yugui Zhang;Rui Cao;Jiangtao Niu;Tian-Tian Bian-Tian;Dingcai Ma;Zhe Wang;Maomao Wang;Xingke Yan
红景天和黄芪在临床上均能改善脾气虚证，尤其是对胃肠功能障碍和免疫功能低下的中医证候有改善作用。本研究旨在比较和揭示HRPCM和ARPCM对SQD大鼠的代谢差异。首先，用高、低剂量中药(12.6 mg/kg)和高剂量中药(12.6 mg/kg)干预SQD大鼠，以进一步评价其干预效果。结果表明，高、中药组均能改善脾病理，提高体重、直肠温度、脾指数、胸腺指数、血清GAS和木糖水平，降低血清IL-2、IL-6和肿瘤坏死因子-α水平。然后用UPLC-Q-TOF-MS研究小鼠血清和脾的代谢差异。结果表明，高、低剂量中药不仅对脾气虚大鼠血清和脾的代谢有调节作用，而且存在一定的差异。其代谢途径主要包括脂质代谢、能量代谢、氨基酸代谢、核苷酸代谢、糖代谢等多种代谢类型。然而，SQD大鼠的代谢产物谱发生了显著变化，主要涉及SQD大鼠甘氨酸合成的异常。QD大鼠HRPCM和ARPCM干预后代谢产物的表达趋势部分相同。有趣的是，对于SQD大鼠，HRPCM和ARPCM在代谢谱上既有相似之处，也有不同之处。差异主要表现在氨基酸代谢中L-谷氨酰胺的合成。
Hedysari Radix Praeparata Cum Melle (HRPCM) and Astragali Radix Praeparata Cum Melle (ARPCM) are capable of improving spleen-qi deficiency (SQD) syndrome especially in the gastrointestinal dysfunction and decreased immunity in traditional Chinese medicine clinically. This study aims to compare and reveal the metabolic differences between HRPCM and ARPCM for SQD rats. Firstly, HRPCM (12.6 g/kg) and ARPCM (12.6 g/kg) were used to intervene SQD rats to further evaluate the effect. The results showed that HRPCM and ARPCM were able to improve the spleen pathology, increase the body weight, the rectal temperature, the spleen index, the thymus index, the levels of GAS andD-xylose in serum, and decrease the levels of IL-2, IL-6 and TNF-α in serum for SQD rats. Then, the studies of metabolic differences in serum and spleen were carried out using UPLC-Q-TOF-MS. The findings emphasized that HRPCM and ARPCM not only regulated metabolic profiling of serum and spleen in SQD rats, but also existed differences. HRPCM and ARPCM regulated metabolic pathways mainly including lipid metabolism, energy metabolism, amino acid metabolism, nucleotide metabolism, sugar metabolism and other types of metabolism for SQD rats. However, the metabolite profiles in SQD rats changed significantly, mainly involving abnormal glycine synthesis occurred in SQD rats. The expression trends of metabolites in HRPCM and ARPCM intervention for SQD rats were partly the same. Interestingly, there are similarities and differences in metabolic profiling between HRPCM and ARPCM for SQD rats. The differences were mainly in the synthesis ofL-glutamine in amino acid metabolism.