The zebrafish (Danio rerio) embryo as a model system for identification and characterization of developmental toxins from marine and freshwater microalgae
The zebrafish (Danio rerio) embryo as a model system for identification and characterization of developmental toxins from marine and freshwater microalgae
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DOI：
10.1016/j.cbpc.2006.07.011
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发表时间：
2007-02-01
影响因子：
3.9
通讯作者：
Schmale, Michael C.
中科院分区：
文献类型：
--
作者：
Berry, John P.;Gantar, Miroslav;Schmale, Michael C.
斑马鱼（Danio rerio）胚胎已成为脊椎动物发育的重要模型。因此，该模型系统在研究抑制或以其他方式干扰发育过程的有毒物质（即发育毒素），包括与人类和环境健康以及生物医学具有潜在相关性的化合物方面具有实用价值。近年来，该系统越来越多地应用于微生物毒素的研究，更具体地说，作为水生动物模型，已被用于研究来自海洋和淡水微藻的毒素，包括那些被归类为所谓的“有害藻华”（HABs）的微藻。我们已经开发了这个系统的鉴定和表征毒素从蓝藻(即。“蓝绿藻”)从佛罗里达大沼泽地和佛罗里达南部和中部的其他淡水资源中分离出来。在这里，我们回顾了该系统在海洋和淡水微藻毒素研究中的应用，并说明了该系统在淡水蓝藻发育毒素的检测、生物测定指导分离和随后表征中的应用。(c) 2006爱思唯尔公司版权所有。
The zebrafish (Danio rerio) ernbryo has emerged as an important model of vertebrate development. As such, this model system is finding utility in the investigation of toxic agents that inhibit, or otherwise interfere with, developmental processes (i.e. developmental toxins), including compounds that have potential relevance to both human and environmental health, as well as biomedicine. Recently, this system has been applied increasingly to the study of microbial toxins, and more specifically, as an aquatic animal model, has been employed to investigate toxins from marine and freshwater microalgae, including those classified among the so-called "harmful algal blooms" (HABs). We have developed this system for identification and characterization of toxins from cyanobacteria (i.e. "blue-green algae") isolated from the Florida Everglades and other freshwater sources in South and Central Florida. Here we review the use of this system as it has been applied generally to the investigation of toxins from marine and freshwater microalgae, and illustrate this utility as we have applied it to the detection, bioassay-guided fractionation and subsequent characterization of developmental toxins from freshwater cyanobacteria. (c) 2006 Elsevier Inc. All rights reserved.