Gas Chromatography-Mass Spectrometry Method for the Determination of Free Amino Acids as Their Dimethyl-tert-butylsilyl (TBDMS) Derivatives in Animal Source Food
Gas Chromatography-Mass Spectrometry Method for the Determination of Free Amino Acids as Their Dimethyl-tert-butylsilyl (TBDMS) Derivatives in Animal Source Food
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DOI：
10.1021/jf2052338
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发表时间：
2012-03-14
影响因子：
6.1
通讯作者：
Antequera, Teresa
中科院分区：
文献类型：
--
作者：
Jimenez-Martin, Estefania;Ruiz, Jorge;Antequera, Teresa
研究了使用 N-甲基-N-(叔丁基二甲基甲硅烷基)三氟乙酰胺一步衍生化程序同时测定 22 种游离氨基酸的适用性及其在 GC-MS 分析六种动物源食品（猪肉、干腌火腿、鸡汤、新鲜奶酪、成熟奶酪和干咸沙丁鱼）中的应用。所有 22 种游离氨基酸衍生物均被正确检测和解析。该方法的重现性 (%RSD) 范围为 1.9-12.2%。分析程序的检测限和定量限分别为 0.01 至 0.46 mg/100 g 干重和 0.02 至 1.55 mg/100 g 干重。校准曲线在 0.1-15.0 mg/100 g 范围内呈线性，相关系数值 (R-2) 为 0.9891 至 0.9983。所有分析的食品均显示出与科学文献中发现的游离氨基酸含量相似的游离氨基酸含量。所提出的用于测定动物源食品中游离氨基酸的 GC-MS 方法可用于常规分析和研究目的。
The suitability of a one-step derivatization procedure using N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide for the simultaneous assay of 22 free amino acids and its application for their analysis in six animal source foods (pork, dry cured ham, chicken stock, fresh cheese, ripened cheese, and dry salted sardine) by GC-MS were studied. All 22 free amino acid derivatives were correctly detected and resolved. Reproducibility (%RSD) of the method was in the range of 1.9-12.2%. Detection and quantitation limits of the analytical procedure ranged from 0.01 to 0.46 mg/100 g dry weight and from 0.02 to 1.55 mg/100 g dry weight, respectively. The calibration curves were linear within the range 0.1-15.0 mg/100 g with correlation coefficient values (R-2) from 0.9891 to 0.9983. All analyzed food products showed free amino acid contents similar to those found in the scientific literature. The proposed GC-MS method for the determination of free amino acids in animal source food can be used in routine for both analytical and research purposes.