Surface-Enhanced Raman Spectroscopy for the Detection of a Metabolic Product in the Headspace Above Live Bacterial Cultures
Surface-Enhanced Raman Spectroscopy for the Detection of a Metabolic Product in the Headspace Above Live Bacterial Cultures
复制标题
表面增强拉曼光谱用于检测活细菌培养物上方顶空的代谢产物
DOI：
10.1002/ange.201808185
复制
发表时间：
2018
影响因子：
--
通讯作者：
Kelly J
中科院分区：
文献类型：
--
作者：
Kelly J
六种细菌培养物上方顶部空间的原位表面增强拉曼光谱显示出化学吸附的甲基硫的强特征条带。这种标记化合物是通过在增强Au或Ag纳米颗粒膜的表面上解离细菌的发酵代谢物二甲基二硫（DMDS）而产生的。加标DMDS的培养基和活培养物的动力学结合图显示，基于Au的基质比基于Ag的基质更适合快速检测细菌。对于E。coliDH5α，顶空Sers检测的灵敏度极限为1.5×107CFU mL−1，相当于接种生长培养基后15 min的检测。 由于代谢产物仅由活菌产生，抗生素（庆大霉素）处理停止了标记峰的正常信号增长。这项工作是朝着细菌感染的快速床边检测和针对细菌的抗生素快速筛选迈出的有希望的一步。
In situ surface‐enhanced Raman spectra of the headspace above cultures of six bacterial species showed strong characteristic bands from chemisorbed methyl sulfide. This marker compound is created by dissociation of dimethyl disulfide (DMDS), a fermentative metabolite of bacteria, on the surface of the enhancing Au or Ag nanoparticle films. Kinetic binding plots of media spiked with DMDS and of live cultures showed that the Au‐based substrates were more suitable for the rapid detection of bacteria than Ag‐based substrates. ForE. coliDH5α, the sensitivity limit for headspace SERS detection was 1.5×107CFU mL−1, which corresponded to detection 15 min after inoculation of the growth medium. Since the metabolites are only produced by viable bacteria, antibiotic (gentamicin) treatment stopped the normal signal growth of the marker peak. This work is a promising step towards rapid bedside detection of bacterial infections and rapid screening of antibiotics against bacteria.