PREVENTION OF PERICYTE GHOST FORMATION IN RETINAL CAPILLARIES OF GALACTOSE-FED DOGS BY ALDOSE REDUCTASE INHIBITORS
PREVENTION OF PERICYTE GHOST FORMATION IN RETINAL CAPILLARIES OF GALACTOSE-FED DOGS BY ALDOSE REDUCTASE INHIBITORS
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DOI：
10.1001/archopht.1988.01060140255036
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发表时间：
1988-08-01
影响因子：
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通讯作者：
KINOSHITA, JH
中科院分区：
文献类型：
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作者：
KADOR, PF;AKAGI, Y;KINOSHITA, JH
早期糖尿病视网膜血管变化的一个显着特征是视网膜毛细血管周细胞（壁细胞）的选择性变性。这些周细胞的损失被认为与毛细血管张力降低、微动脉瘤的形成和血管扩张有关。醛糖还原酶在糖尿病视网膜病变进展中的作用已在年龄和性别匹配的小猎犬中进行了研究，这些小猎犬喂食 30% 半乳糖饮食，并添加或不添加醛糖还原酶抑制剂索宾尼或 M79175。定期将每组狗的眼睛摘除，并用胰蛋白酶消化的扁平制剂检查它们的视网膜毛细血管。在临床出现视网膜变化之前，半乳糖喂养 21 个月后，四分之三的狗和所有狗 24 个月后，在眼睛中观察到周细胞影形成。许多含有周细胞影的毛细血管表现出内皮细胞和无细胞血管的明显增殖。在正常对照（九只中的零只）或用醛糖还原酶抑制剂治疗的半乳糖喂养的狗（16只中的零只）的视网膜中没有观察到周细胞鬼影或异常发现。这些发现表明醛糖还原酶抑制剂可以防止实验性视网膜病变中周细胞影的形成和其他随后的毛细血管变化。
A distinguishing feature of early diabetic retinal vascular changes is the selective degeneration of pericytes (mural cells) from the retinal capillary vessels. Loss of these pericytes has been proposed to be associated with decreased capillary tonicity, the formation of microaneurysms, and vessel dilation. The role of aldose reductase in the progression of diabetic retinopathy has been investigated in age- and sex-matched beagles fed a 30% galactose diet with or without the aldose reductase inhibitors sorbinil or M79175. Eyes were periodically enucleated from dogs in each group and their retinal capillaries were examined as trypsin-digested flat preparations. Before the clinical appearance of retinal changes, pericyte ghost formation was observed in the eyes of three fourths of the dogs after 21 months and all of the dogs after 24 months of galactose feeding. Many of the capillaries containing pericyte ghosts demonstrated an apparent proliferation of endothelial cells and acellular vessels. No pericyte ghosts or abnormal findings were observed in retinas from either normal control (zero of nine) or galactose-fed dogs treated with aldose reductase inhibitors (zero of 16). These findings indicate that aldose reductase inhibitors can prevent the formation of pericyte ghosts and other subsequent capillary changes in experimental retinopathy.