BASOPHILIC LEUKOCYTES IN ALLERGIC CONTACT-DERMATITIS
BASOPHILIC LEUKOCYTES IN ALLERGIC CONTACT-DERMATITIS
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DOI：
10.1084/jem.135.2.235
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发表时间：
1972-01-01
影响因子：
15.3
通讯作者：
MIHM, MC
中科院分区：
文献类型：
--
作者：
DVORAK, HF;MIHM, MC
实验动物中多种细胞介导的超敏反应包括嗜碱性白细胞的显着浸润。这种形式的反应性被称为皮肤嗜碱性粒细胞超敏反应，并且在不使用完全弗氏佐剂的情况下完成对几种类型抗原的敏化时受到青睐。使用允许识别嗜碱性白细胞的形态学技术在人类中寻找类似类型的超敏反应。研究人员对 8 名因多种过敏原而患有过敏性接触性皮炎的个体进行了研究，其中 6 名个体出现了典型的红斑、水肿和表皮水泡接触反应。这些个体的 3 天活组织检查的显微结果与结核菌素过敏的经典描述显着不同，除了单核细胞和特征性表皮变化外，还显示嗜碱性白细胞大量浸润，以及血管通透性改变、血管压缩、真皮水肿和纤维蛋白沉积的证据。对一个人进行的连续活检可以对斑贴试验后连续间隔展开的微观病理学进行分析。最初的病变包括血管周围淋巴细胞的积聚；随后是嗜碱性粒细胞和嗜酸性粒细胞的涌入。这些发现将人类接触过敏与动物皮肤嗜碱性粒细胞过敏的平行反应联系起来，并为细胞免疫反应的异质性提供了进一步的证据。这些数据与以下假设一致：在局部测试位点，致敏淋巴细胞和抗原之间的相互作用是吸引嗜碱性粒细胞的原因。它们还直接暗示延迟型反应中的凝血系统，并表明细胞免疫与嗜碱性粒细胞结合的同型细胞营养抗体介导的反应之间可能存在协同关系。
A variety of cell-mediated hypersensitivity reactions in experimental animals include a prominent infiltrate of basophilic leukocytes. This form of reactivity has been designated cutaneous basophil hypersensitivity and is favored when sensitization to several types of antigen is accomplished without the use of complete Freund's adjuvant.A similar type of hypersensitivity response was sought in man using morphologic techniques which permit identification of basophilic leukocytes. Eight individuals with allergic contact dermatitis to a variety of allergens were studied and six of these developed typical contact reactions with erythema, edema, and epidermal vesicles. The microscopic findings in 3-day biopsies from these individuals differed significantly from classic descriptions of tuberculin hypersensitivity and showed, in addition to mononuclear cells and the characteristic epidermal changes, a substantial infiltrate of basophilic leukocytes and evidence of altered vascular permeability with vascular compaction, dermal edema, and fibrin deposition. Serial biopsies from one individual permitted analysis of the microscopic pathology as it unfolded at successive intervals after patch test. The initial lesion consisted of perivascular accumulations of lymphocytes; this was followed by an influx of basophils and, subsequently, of eosinophils.These findings associate contact allergy in man with the parallel reactions of cutaneous basophil hypersensitivity in animals and provide further evidence for the heterogeneity of the cellular immune response. The data are consistent with the hypothesis that interaction between sensitized lymphocytes and antigen, at a local test site, is responsible for the attraction of basophils. They also directly implicate the clotting system in delayed-type reactions and suggest the possibility of a synergistic relationship between cellular immunity and reactions mediated by basophil-bound, homocytotrophic antibody.