Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain
Distinct spatio-temporal expression of ABCA and ABCG transporters in the developing and adult mouse brain
复制标题
DOI：
10.1111/j.1471-4159.2005.03369.x
复制
发表时间：
2005-10-01
影响因子：
4.7
通讯作者：
Terasaki, T
中科院分区：
文献类型：
--
作者：
Tachikawa, M;Watanabe, M;Terasaki, T
利用小鼠脑原位杂交技术，我们分析了atp结合盒（ABC）转运蛋白亚家族ABCA1-4和7以及ABCG1、2、4、5和8基因表达的发育变化。在胚胎脑中，ABCA1和A7在心室区（或生发区）高表达，而ABCA2， A3和G4在套膜区（或分化区）富集。出生后，ABCA1在灰质、白质和脉络膜丛中均有检测。另一方面，ABCA2、A3和A7分布在灰质中。此外，ABCA2的显著上调发生在14日龄的白质中，此时已知各种髓鞘蛋白基因上调。与此相反，ABCA4在整个发育过程中对脉络膜丛具有选择性。ABCG1在灰质和白质中均有表达，而abc4仅在灰质中表达。ABCG2在全脑各阶段均呈弥漫性弱表达。免疫组化显示ABCG2在脑毛细血管管腔膜上优先表达。ABCG5和G8在各阶段几乎未见表达信号。ABCA和G转运体的时空表达差异可能反映了它们在发育和成人大脑中不同的细胞表达，可能是为了调节和维持脑内脂质稳态。
Using in situ hybridization for the mouse brain, we analyzed developmental changes in gene expression for the ATP-binding cassette (ABC) transporter subfamilies ABCA1-4 and 7, and ABCG1, 2, 4, 5 and 8. In the embryonic brains, ABCA1 and A7 were highly expressed in the ventricular (or germinal) zone, whereas ABCA2, A3 and G4 were enriched in the mantle (or differentiating) zone. At the postnatal stages, ABCA1 was detected in both the gray and white matter and in the choroid plexus. On the other hand, ABCA2, A3 and A7 were distributed in the gray matter. In addition, marked up-regulation of ABCA2 occurred in the white matter at 14 days-of-age when various myelin protein genes are known to be up-regulated. In marked contrast, ABCA4 was selective to the choroid plexus throughout development. ABCG1 was expressed in both the gray and white matters, whereas ABCG4 was confined to the gray matter. ABCG2 was diffusely and weakly detected throughout the brain at all stages examined. Immunohistochemistry of ABCG2 showed its preferential expression on the luminal membrane of brain capillaries. Expression signals for ABCG5 and G8 were barely detected at any stages. The distinct spatio-temporal expressions of individual ABCA and G transporters may reflect their distinct cellular expressions in the developing and adult brains, presumably, to regulate and maintain lipid homeostasis in the brain.