Complement fixation antibody test for human nocardiosis
Complement fixation antibody test for human nocardiosis
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人诺卡氏菌病补体结合抗体检测
DOI：
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发表时间：
1978
影响因子：
9.4
通讯作者：
D. Stevens
中科院分区：
文献类型：
--
作者：
J. Shainhouse;A. Pier;D. Stevens
在16例有组织学和/或培养证据的诺卡氏菌感染患者中，有13例检测到补体结合抗体。所使用的抗原是由星状核菌培养物分泌到液体培养基中的可溶性物质的滤液。在3名麻风病患者中有3名患者和5名结核病患者中有2名患者发现了明显的假阳性反应--结果与以前报道的一些方法相似。星状核菌和分枝杆菌有共同的抗原。只有肺结核的假阳性才被认为是诊断问题。26例其他感染患者和41例未感染患者的血清均未见反应。虽然以前的诺卡氏菌血清学诊断方法的灵敏度约为50%，但我们的总体灵敏度(81%)更好，包括免疫功能低下的诺卡菌病患者的11项阳性测试中的9项(与以前的方法相比，这是假阴性反应的来源)。
Complement-fixing antibody was detected in 13 of 16 patients with histological and/or culture evidence of infection with Nocardia species. The antigen used was a filtrate of soluble substances secreted into liquid growth medium by cultures of N. asteroides. Apparent false positives reactions were found in three of three patients with leprosy and two of five patients with tuberculosis--results similar to some previously reported methods. N. asteroides and mycobacteria share antigens. Only the false positives with tuberculosis are considered a diagnostic problem. No reactions were obtained with sera from 26 patients with other infections and 41 unifected individuals. Whereas previous nocardia serodiagnostic methods have a sensitivity of approximately 50%, our overall sensitivity (81%) compares favorably and included 9 of 11 positive tests in immunocompromised nocardiosis patients (a source of false negative reactions with previous methods).