Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device.
Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device.
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DOI：
10.1021/acs.analchem.6b00837
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发表时间：
2016-06-07
影响因子：
7.4
通讯作者：
Ros A
中科院分区：
文献类型：
--
作者：
Luo J;Muratore KA;Arriaga EA;Ros A
微米和亚微米粒子的有效分离在纳米技术和生物应用材料的制备和分析中非常有用。在这里，我们展示了一种非直观但高效的µm和亚µm胶体颗粒和细胞器的分离机制，利用微流体装置中在电动力和介电力周期性作用下的非线性柱阵列中的颗粒传输。我们揭示了确定性粒子迁移与平均施加力相反的机制，这是确定性绝对负迁移率（dANM）的典型特征，而对于较小的粒子则获得正常响应。在数值模拟中对dANM和正常迁移的共存进行了表征和优化，随后在微流控装置中实现，与之前的ANM系统相比，其迁移速度至少提高了2个数量级。我们还为小鼠肝脏线粒体诱导了dANM，并设想这里描述的分离机制为未来细胞器、纳米颗粒和蛋白质纳米晶体的分离提供了所需的尺寸选择性。
Efficient separations of particles with micron and submicron dimensions are extremely useful in preparation and analysis of materials for nanotechnological and biological applications. Here, we demonstrate a nonintuitive, yet efficient, separation mechanism for µm and subµm colloidal particles and organelles, taking advantage of particle transport in a nonlinear post array in a microfluidic device under the periodic action of electrokinetic and dielectrophoretic forces. We reveal regimes in which deterministic particle migration opposite to the average applied force occurs for a larger particle, a typical signature of deterministic absolute negative mobility (dANM), whereas normal response is obtained for smaller particles. The coexistence of dANM and normal migration was characterized and optimized in numerical modeling and subsequently implemented in a microfluidic device demonstrating at least 2 orders of magnitude higher migration speeds as compared to previous ANM systems. We also induce dANM for mouse liver mitochondria and envision that the separation mechanisms described here provide size selectivity required in future separations of organelles, nanoparticles, and protein nanocrystals.