A model of multiple myeloma: culture of 5T33 murine myeloma cells and evaluation of tumorigenicity in the C57BL/KaLwRij mouse.
A model of multiple myeloma: culture of 5T33 murine myeloma cells and evaluation of tumorigenicity in the C57BL/KaLwRij mouse.
复制标题
DOI：
10.1038/bjc.1992.415
复制
发表时间：
1992-12
影响因子：
8.8
通讯作者：
Turner, J H
中科院分区：
文献类型：
--
作者：
Manning, L S;Berger, J D;O'Donoghue, H L;Sheridan, G N;Claringbold, P G;Turner, J H
5 T33多发性骨髓瘤是C57 BL/KaLwRij小鼠中自发产生的一系列可移植性鼠骨髓瘤之一。本研究描述了5 T33小鼠骨髓瘤体外培养细胞系的建立和表征，包括其形态、生长速率、副蛋白（IgG 2b）表达和在同基因动物中的致瘤性。5 T33细胞系已连续培养超过10个月，并已达到超过34代。在培养中，5 T33骨髓瘤作为单个细胞或在粘附基质细胞层上的松散粘附细胞的小簇中生长。最大倍增时间约为25小时，超过90%的细胞表达胞质IgG 2b副蛋白。培养的5 T33骨髓瘤细胞在C57 BL/KaLwRij小鼠中具有高度致瘤性，早在肿瘤接种后第36天，就有500个细胞诱导瘫痪和死亡。肿瘤发展的动力学和IgG 2b副蛋白的检测具有剂量依赖性。静脉内接种5 × 10（5）个培养的5 T33骨髓瘤细胞后两周，在骨髓中很容易识别出肿瘤细胞。到肿瘤接种后3周，在整个动物的各种组织中发现5 T33骨髓瘤细胞。目前正在进行研究，以确定该细胞系对各种治疗方式的敏感性。
The 5T33 multiple myeloma is one of a series of transplantable murine myelomas arising spontaneously in C57BL/KaLwRij mice. This study describes the establishment and characterisation of the 5T33 murine myeloma in vitro as a cultured cell line in terms of its morphology, growth rate, expression of paraprotein (IgG2b) and tumorigenicity in syngeneic animals. The 5T33 cell line has been in continuous culture for over 10 months and has achieved more than passage 34. In culture, 5T33 myeloma grows as single cells or in small clusters of loosely adherent cells on an adherent stromal cell layer. Maximum doubling time is approximately 25 h, and over 90% of the cells express cytoplasmic IgG2b paraprotein. The cultured 5T33 myeloma cells are highly tumorigenic in C57BL/KaLwRij mice with as few as 500 cells inducing paralysis and death as early as day 36 post-tumour inoculation. Kinetics of tumour development and detection of IgG2b paraprotein are dose dependent. Two weeks following intravenous inoculation of 5 x 10(5) cultured 5T33 myeloma cells, tumour cells were readily identified in the bone marrow. By 3 weeks post-tumour inoculation, 5T33 myeloma cells were found in various tissues throughout the animal. Studies are now underway to determine the sensitivity of this cell line to various therapeutic modalities.