Olfactory marker protein contributes to the evaluation of odour values by olfactory glomerular processing
Olfactory marker protein contributes to the evaluation of odour values by olfactory glomerular processing
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DOI：
10.1016/j.neulet.2020.135445
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发表时间：
2020-11-20
影响因子：
2.5
通讯作者：
Nakashima, Noriyuki
中科院分区：
文献类型：
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作者：
Nakashima, Akiko;Nakagawa, Taku;Nakashima, Noriyuki
嗅觉起源于表达嗅觉标记蛋白（OMP）的嗅觉受体神经元（ORN）。OMP缺陷导致各种行为表型，表明由于ORN反应受损导致嗅觉功能障碍。最近，OMP被证明通过缓冲嗅觉cAMP信号来维持强烈的嗅觉。然而，OMP对嗅觉行为的影响，其评估需要时间来评估气味值，仍然在很大程度上unexplained. There，我们研究了行为的杂合子OMP+/GFP（HET）小鼠与同源GFP敲入OMP缺陷OMP（GFP/ GFP）（KI）小鼠在嗅觉调查的气味与不同的价值观。当出现含有有机气味的拭子时，HET和KI小鼠都迅速接近并在测试期间逐渐习惯地研究拭子。然而，当出现另一种类似的气味时，KI小鼠对新拭子的研究远不如HET小鼠深入。接下来，将小鼠放置在测试室的一个角落中具有厌恶气味源的室中。KI小鼠比HET小鼠更频繁地接近含有厌恶气味源的隔室。最后，我们训练小鼠通过利用奖惩值将两种气味与解决方案联系起来。HET小鼠保持接近奖励相关的气味，而KI小鼠最初接近奖励相关的气味，偶尔转向惩罚相关的气味源，并最终留在奖励气味隔室。组织学上，表达c-Fos的肾小球细胞较少，更广泛地分布在肾小球周围的KI小鼠比HET mice.In结论，OMP有助于评价气味值的肾小球处理过程中的嗅觉调查任务。
Olfaction starts from olfactory receptor neurons (ORNs) that express olfactory marker protein (OMP). OMP deficit results in various behavioural phenotypes indicating olfactory dysfunction due to the impaired responses of ORNs. Recently, OMP was demonstrated to maintain strong olfaction by buffering olfactory cAMP signalling. However, the impact of OMP on olfaction behaviours, the assessment of which requires time to evaluate odour values, remains largely unexplained.Here, we examined the behaviour of heterozygous OMP+/GFP (HET) mice vs. homologous GFP-knock-in OMPdeficient OMP (GFP/ GFP) (KI) mice during the olfactory investigation of odours with different values. When a swab containing an organic odour was presented, both HET and KI mice swiftly approached and investigated the swab with gradual habituation over test sessions. However, when another similar odour was presented, KI mice investigated the new swab much less intensively than HET mice. Next, mice were placed in a chamber with an aversive odour source in one corner of a test chamber. KI mice more frequently approached the compartment containing the aversive odour source than HET mice. Finally, we trained mice to associate two odours with solutions by utilizing reward-penalty values. HET mice stayed close to the reward-associated odour, while KI mice initially approached the reward-associated odour, occasionally turned towards the penalty-associated odour source and eventually stayed in the reward-odour compartment. Histologically, c-Fos-expressing juxtaglomerular cells were fewer and more broadly distributed around glomeruli in KI mice than HET mice.In conclusion, OMP contributes to the evaluation of odour values by glomerular processing during an olfactory investigation task.