PREIMPLANTATION EMBRYO DEVELOPMENT INVITRO - COOPERATIVE INTERACTIONS AMONG EMBRYOS AND ROLE OF GROWTH-FACTORS
PREIMPLANTATION EMBRYO DEVELOPMENT INVITRO - COOPERATIVE INTERACTIONS AMONG EMBRYOS AND ROLE OF GROWTH-FACTORS
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DOI：
10.1073/pnas.87.12.4756
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发表时间：
1990-06-01
影响因子：
11.1
通讯作者：
DEY, SK
中科院分区：
文献类型：
--
作者：
PARIA, BC;DEY, SK
我们已经建立了一个模型，显示合作的相互作用，植入前胚胎和生长因子的作用，他们的发展和增长。在25 μ l微滴中单独培养的双细胞小鼠胚胎与在5或10个微滴的组中培养的那些相比，发育差于胚泡并且每个胚泡的细胞数较低。通过加入表皮生长因子（EGF）或转化生长因子α，可显著改善单一培养胚胎的不良发育。或β 1（TGF-α.或TGF-β 1）添加到培养基中。主要受这些处理影响的胚胎发育阶段在八细胞/桑椹胚和囊胚之间。此外，囊胚发育的8细胞胚胎培养组或单独在EGF的存在下，表现出较高的发病率的孵化与单独培养的EGF的情况下相比。在八细胞/桑椹胚和胚泡阶段的胚胎细胞表面上EGF受体的检测表明EGF或TGF-α的有益作用。对植入前胚胎发育和囊胚功能的影响。胰岛素样生长因子I（IGF-I）对胚胎发育无影响。为了进一步记录胚胎之间的合作相互作用，培养基的体积增加一倍至50 μ l。这种培养体积的增加对单独培养的胚胎的发育更加不利。然而，这种有害作用被EGF显著地逆转，并且被EGF和TGF-β 1的组合逆转得甚至更显著，而不是被单独的TGF-β 1逆转。虽然TGF-β 1加IGF-I引起胚胎发育的适度改善，但反应不如单独EGF所显示的那么大。此外，IGF-I对EGF诱导的胚胎发育没有累加效应。这项研究提供了明确的证据表明，胚胎和/或生殖道来源的特定生长因子以自分泌/旁分泌方式参与植入前胚胎发育和囊胚功能。
We have established a model that shows cooperative interaction among preimplantation embryos and the role of growth factors on their development and growth. Two-cell mouse embryos cultured singly in 25-.mu.l microdrops had inferior development to blastocysts and lower cell numbers per blastocyst compared with those cultured in groups of 5 or 10. The inferior development of singly cultured embryos was markedly improved by addition of epidermal growth factor (EGF) or transforming growth factor .alpha. or .beta.1 (TGF-.alpha. or TGF-.beta.1) to the culture medium. The stage of embryonic development, primarily affected by these treatments, was between eight-cell/morula and blastocyst. Furthermore, blastocysts developed from eight-cell embryos cultured in groups or singly in the presence of EGF showed a higher incidence of zona hatching compared with those cultured singly in the absence of EGF. Detection of EGF receptors on the embryonic cell surface at eight-cell/morula and blastocyst stages suggest beneficial effects of EGF or TGF-.alpha. on preimplantation embryos development and blastocyst functions. Insulin-like growth factor I (IGF-I) had no influence on embryo development. To further document the cooperative interactions among embryos, the volume of the culture medium was doubled to 50.mu.l. This increase in culture volume was even more detrimental to the development of singly cultured embryos. However, this detrimental effect was significantly reversed by EGF and reversed even more markedly by a combination of EGF and TGF-.beta.1 but not by TGF-.beta.1 alone. Although TGF-.beta.1 plus IGF-I caused a modest improvement of embryo development, the response was not as great as shown by EGF alone. Furthermore IGF-I had no additive effect on EGF-induced embryonic development. The study presents clear evidence that specific growth factors of embryonic and/or reproductive tract origin participate in preimplantation embryo development and blastocyst functions in an autocrine/paracrine manner.