ABSENCE OF TRANSOVARIAL TRANSMISSION OF BLUETONGUE VIRUS IN CULICOIDES-VARIIPENNIS - IMMUNOGOLD LABELING OF BLUETONGUE VIRUS-ANTIGEN IN DEVELOPING OOCYTES FROM CULICOIDES-VARIIPENNIS (COQUILLETT)
ABSENCE OF TRANSOVARIAL TRANSMISSION OF BLUETONGUE VIRUS IN CULICOIDES-VARIIPENNIS - IMMUNOGOLD LABELING OF BLUETONGUE VIRUS-ANTIGEN IN DEVELOPING OOCYTES FROM CULICOIDES-VARIIPENNIS (COQUILLETT)
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DOI：
10.1016/0300-9629(90)90036-r
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发表时间：
1990-01-01
影响因子：
2.3
通讯作者：
MECHAM, JO
中科院分区：
文献类型：
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作者：
NUNAMAKER, RA;SIEBURTH, PJ;MECHAM, JO
1.用含蓝舌病病毒（BTV）血清型11（BTV-11）的血粉喂养变翅库蠓，然后每隔4天喂4次非感染性血粉。2.在每次吸血前收集卵并饲养至成虫。3.将来自每批蛋的后代孵育14天（20 ℃）。C，40- 60%RH）。4.从几个亲蝇卵母细胞切片免疫电镜。5.通过空斑试验检测的32%的母鸟对BTV呈阳性。6.所有993只子代果蝇均为BTV阴性。7. BTV抗原在发育卵母细胞的卵黄蛋白体和卵黄膜中密集。
1. Culicoides variipennis midges were fed on a blood meal containing bluetongue virus (BTV) serotype 11 (BTV-11) and on four subsequent non-infective blood meals at 4-day intervals. 2. Eggs were collected before each blood-feeding and reared to adults. 3. Progeny from each egg batch were incubated for 14 days (20.degree. C, 40-60% RH) before plaque assay. 4. Oocytes from several parent flies were sectioned for immunoelectron microscopy. 5. Thirty-two percent of the parent females tested by plaque assay were positive for BTV. 6. All 993 progeny flies were negative for BTV. 7. BTV antigen was dense in proteid yolk bodies and in the vitelline membrane of the developing oocytes.