Progesterone initiation of the human sperm acrosome reaction: the obligatory increase in intracellular calcium is independent of the chloride requirement.
Progesterone initiation of the human sperm acrosome reaction: the obligatory increase in intracellular calcium is independent of the chloride requirement.
复制标题
黄体酮引发人类精子顶体反应：细胞内钙的强制性增加与氯的需求无关。
DOI：
10.1016/0303-7207(94)90238-0
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发表时间：
1994
影响因子：
4.1
通讯作者：
Meizel,S
中科院分区：
文献类型：
--
作者：
Turner,KO;Garcia,MA;Meizel,S
胆甾酮引发的人类精子顶体反应（AR）需要细胞内Ca 2+（[Ca 2 +]i）和细胞外Cl−升高，并通过一个独特的类固醇受体/Cl−通道明显增加Cl−通量，该通道类似于但不完全相同于GABAA/C1−通道复合物。本研究使用载有fura-2的人精子、GABAA/CL−通道阻断剂（印防己毒素和硫酸双烯醇酮）和含Cl−和缺乏Cl−的培养基，以确定胆甾酮介导的[Ca 2 +] i增加是否依赖于Cl−需求。在含Cl−和缺乏Cl−的培养基中，胆甾酮介导的[Ca 2 +] i增加之间没有显著差异。印防己毒素不能显著抑制孕酮介导的[Ca ~（2+）]i的增加，硫酸双烯醇酮使[Ca ~（2+）] i增加到与孕酮相同的程度。这些结果有力地表明，AR所必需的[Ca 2 +] i的增加与AR的Cl-需求无关，并且可以通过两种不同的精子质膜孕酮受体的存在来解释。
The progesterone-initiated human sperm acrosome reaction (AR) requires a rise in intracellular Ca2+([Ca2+]i), extracellular Cl−and apparently increased Cl−flux through a unique steroid receptor/Cl−channel resembling but not identical to a GABAA/C1−channel complex. The present study uses fura-2 loaded human sperm, GABAA/CL−channel blockers (picrotoxin and pregnenolone sulfate) and Cl−-containing and Cl−-deficient media to determine whether the progesterone-mediated increase in [Ca2+]iis dependent on the Cl−requirement. There was no significant difference between the progesterone-mediated increases of [Ca2+]iobtained in Cl−-containing and Cl−-deficient media. Picrotoxin did not significantly inhibit the progesterone-mediated increase in [Ca2+]i, and pregnenolone sulfate increased [Ca2+]ito the same extent as progesterone. These results strongly suggest that the increase in [Ca2+]iessential to the AR is independent of the AR Cl−requirement and could be explained by the existence of two different sperm plasma membrane progesterone receptors.