Molecular epidemiology of VapA-positive Rhodococcus equi in thoroughbred horses in Kagoshima, Japan.
Molecular epidemiology of VapA-positive Rhodococcus equi in thoroughbred horses in Kagoshima, Japan.
复制标题
日本鹿儿岛纯种马中 VapA 阳性马红球菌的分子流行病学。
DOI：
10.1292/jvms.64.715
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Takai
中科院分区：
文献类型：
--
作者：
T. Yuyama;Shigemoto Yusa;K. Yoshizumi;S. Yamano;S. Murata;T. Hirose;R. Osanai;Y. Onishi;Satoshi Osato;Chie Sasaki;Y. Sasaki;T. Kakuda;S. Tsubaki;S. Takai
强毒R.从日本鹿儿岛5个马场的13匹纯种马驹及其母驴粪便分离的15 ~ 17 kDa抗原（VapA）马立克次体（R. equi）中分离出15 ~ 17 kDa抗原的马立克次体（R. equi），并通过限制性酶切分析VapA阳性分离株的质粒谱，比较R. equi毒力质粒的基因型变异。马属分离物来自日本。总计，来自13匹马驹粪便的886株分离株中有218株（24.6%）和来自其母驴粪便的104株分离株中有13株（12.5%）表现出VapA阳性R。等。用EcoR Ⅰ、EcoT 22 Ⅰ和HindIII三种内切酶对231株马驹和母畜强毒分离株的质粒DNA进行酶切分析，将其分为3种类型：172株含有90-kb Ⅰ型质粒，57株含有90-kb Ⅲ型质粒，2株含有90-kb Ⅳ型质粒。这项研究表明，在鹿儿岛的纯种马驹VapA阳性菌株中发现的毒力质粒的分布的地理特征。
The prevalence of virulent R. equi having 15- to 17-kDa antigens (VapA) in fecal isolates from 13 thoroughbred foals and their dams on 5 farms in Kagoshima, Japan, and the plasmid profiles of VapA-positive isolates by restriction fragment digestion patterns were investigated to compare the genotypic variation among virulence plasmids of R. equi isolates from Japan. In total, 218 (24.6%) of 886 isolates from the feces of the 13 foals and 13 (12.5%) of 104 isolates from the feces of their dams demonstrated VapA-positive R. equi. Plasmid DNA preparations of 231 virulent isolates from foals and dams were analyzed by restriction enzyme digestion with endonucleases EcoRI, EcoT22I and HindIII and were divided into 3 types: 172 isolates contained a 90-kb type I plasmid, 57 contained a 90-kb type III plasmid and 2 contained a 90-kb type IV plasmid. This study demonstrates a geographic character in the distribution of virulence plasmids found in VapA-positive isolates from thoroughbred foals in Kagoshima.