Target-induced aptamer release strategy based on electrochemical detection of staphylococcal enterotoxin B using GNPs-ZrO2-Chits film
Target-induced aptamer release strategy based on electrochemical detection of staphylococcal enterotoxin B using GNPs-ZrO2-Chits film
复制标题
基于 GNPs-ZrO2-Chits 薄膜电化学检测葡萄球菌肠毒素 B 的靶标诱导适体释放策略
DOI：
10.1016/j.colsurfb.2014.04.028
复制
发表时间：
2014-08-01
影响因子：
5.8
通讯作者：
Xie, Guoming
中科院分区：
文献类型：
--
作者：
Deng, Renni;Wang, Li;Xie, Guoming
A novel electrochemical aptasensor was developed for ultrasensitive detection of staphylococcal enterotoxin B (SEB) by combining signal amplification and target-induced aptamer release strategy. A gold electrode was modified with a nanocomposite made of gold nanoparticles reduced in situ, zirconia nanoparticles, and chitosan. The SEB aptamer was hybridized by anchoring the capture probe on the modified gold electrode surface through Au-S binding. In the presence of SEB, the capture probe-aptamer duplex was compelled to open, releasing the aptamer from the electrode. The resulting single-strand capture probe was hybridized with a biotinylated detection probe and labeled with streptavidin-horseradish peroxidase, producing an ultrasensitive enzyme-catalyzed electrochemical signal. Under optimal conditions, the amperometric responses were proportional to the SEB concentrations ranging from 2 to 512 ng mL(-1), with a detection limit of as low as 0.24 ng mL(-1) (S/N = 3). The aptasensor exhibited good stability, outstanding reproducibility, and high selectivity. The as-prepared aptasensor was used to analyze SEB in human serum specimens, and validated through enzyme-linked immunosorbent assay method. Analytical results suggest that the developed assay is a promising alternative approach for detecting SEB in clinical diagnosis. (C) 2014 Elsevier B.V. All rights reserved.
