Effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and antioxidant capacity
Effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and antioxidant capacity
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DOI：
10.1111/asj.13613
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发表时间：
2021
影响因子：
2
通讯作者：
Feng Gao
中科院分区：
文献类型：
--
作者：
Yang Zi;Chi Ma;Huimin Li;Suting Shen;Yingchun Liu;Ming Li;Feng Gao
本研究探讨了妊娠晚期宫内生长受限对绵羊胎儿肾功能和肾脏抗氧化能力的影响。 18只怀孕母羊随机分为对照组（CG，随意采食，0.67 MJ ME·BW−0.75·day−1，n= 6）、限制组1（RG1，0.18 MJ ME·BW−0.75·day−1，n= 6）和限制组2（RG2，0.33 MJ） ME·BW−0.75·day−1,n=6)。 140天时采集胎儿血液、尿囊液和肾组织，测定胎儿肾功能和肾脏抗氧化能力。结果显示，RG1组胎儿体重、肾脏重量、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、水通道蛋白-2（AQP-2）和水通道蛋白-3（AQP-3）以及总抗氧化能力（T-AOC）较CG1组降低（P<0.05），但β2-微球蛋白（β2-MG）、 RG1组的胱抑素C（Cys-C）、滤钠排泄分数（FENa）、丙二醛（MDA）和羟自由基（OH）增加（P<0.05）。孕晚期母体营养不良导致羊胎儿肾脏生长受损、抗氧化失衡、肾小球超滤功能障碍以及肾小管重吸收功能。
This study investigated the effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and renal antioxidant capacity. Eighteen ewes pregnant were randomly divided into control group (CG, ad libitum, 0.67 MJ ME·BW−0.75·day−1,n= 6), restricted group 1 (RG1, 0.18 MJ ME·BW−0.75·day−1,n= 6), and restricted group 2 (RG2, 0.33 MJ ME·BW−0.75·day−1,n= 6). At 140 days, the fetal blood, allantoic fluid and kidney tissue were collected to determinate fetal renal function and renal antioxidant capacity. The results showed that the fetal weight, kidney weight, superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), aquaporin‐2 (AQP‐2) and aquaporin‐3 (AQP‐3), and total antioxidant capacity (T‐AOC) in RG1 group were decreased compared with the CG (P< 0.05), but the contents of β2‐Microglobulin (β 2‐MG), cystatin C (Cys‐C), filtered sodium excretion fraction (FENa), malondialdehyde (MDA), and hydroxyl radical (OH) in RG1 group were increased (P< 0.05). The impaired ovine fetal renal growth, antioxidant imbalance and dysfunction of glomerulus ultrafiltration, and the renal tubules reabsorption were induced by maternal malnutrition during late pregnancy.