Inhibitory effect of baicalin and baicalein on ovarian cancer cells.
Inhibitory effect of baicalin and baicalein on ovarian cancer cells.
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DOI：
10.3390/ijms14036012
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发表时间：
2013-03-15
影响因子：
5.6
通讯作者：
Chen YC
中科院分区：
文献类型：
--
作者：
Chen J;Li Z;Chen AY;Ye X;Luo H;Rankin GO;Chen YC
卵巢癌是西方世界女性死亡的主要原因之一。黄芩苷和黄芩素是天然存在的黄酮类化合物，存在于一些中药植物的根和叶中，被认为具有抗氧化活性和可能的抗血管生成、抗癌、抗焦虑、抗炎和神经保护活性。选用卵巢癌细胞株OVCAR-3和CP-70及正常卵巢癌细胞株IOSE-364，研究黄芩苷和黄芩素对卵巢癌细胞的抑制作用。黄芩苷和黄芩素在两种卵巢癌细胞系中均抑制卵巢癌细胞活力，黄芩苷的LD 50值范围为45-55 μM，黄芩素为25-40 μM。另一方面，两种化合物对正常卵巢细胞活力的抑制作用较小，黄芩苷的LD 50值为177 μM，黄芩素为68 μM。黄芩苷降低VEGF（20 μM）、cMyc（80 μM）和NFkB（20 μM）的表达;黄芩素降低VEGF（10 μM）、HIF-1α（20 μM）、cMyc（20 μM）和NFkB（40 μM）的表达。因此黄芩素在抑制两种卵巢癌细胞系中的癌细胞活力和VEGF、HIF-1α、cMyc和NFκB的表达方面更有效。黄芩素可能通过抑制VEGF、HIF-1α、cMyc和NFκB等促癌基因的表达而抑制癌细胞活力。总的来说，这项研究表明，黄芩素和黄芩苷显着抑制卵巢癌细胞的活力，而通常对正常细胞的影响较小。它们具有化学预防和治疗卵巢癌的潜力。
Ovarian cancer is one of the primary causes of death for women all through the Western world. Baicalin and baicalein are naturally occurring flavonoids that are found in the roots and leaves of some Chinese medicinal plants and are thought to have antioxidant activity and possible anti-angiogenic, anti-cancer, anxiolytic, anti-inflammatory and neuroprotective activities. Two kinds of ovarian cancer (OVCAR-3 and CP-70) cell lines and a normal ovarian cell line (IOSE-364) were selected to be investigated in the inhibitory effect of baicalin and baicalein on cancer cells. Largely, baicalin and baicalein inhibited ovarian cancer cell viability in both ovarian cancer cell lines with LD50 values in the range of 45–55 μM for baicalin and 25–40 μM for baicalein. On the other hand, both compounds had fewer inhibitory effects on normal ovarian cells viability with LD50 values of 177 μM for baicalin and 68 μM for baicalein. Baicalin decreased expression of VEGF (20 μM), cMyc (80 μM), and NFkB (20 μM); baicalein decreased expression of VEGF (10 μM), HIF-1α (20 μM), cMyc (20 μM), and NFkB (40 μM). Therefore baicalein is more effective in inhibiting cancer cell viability and expression of VEGF, HIF-1α, cMyc, and NFκB in both ovarian cancer cell lines. It seems that baicalein inhibited cancer cell viability through the inhibition of cancer promoting genes expression including VEGF, HIF-1α, cMyc, and NFκB. Overall, this study showed that baicalein and baicalin significantly inhibited the viability of ovarian cancer cells, while generally exerting less of an effect on normal cells. They have potential for chemoprevention and treatment of ovarian cancers.
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乳腺癌和卵巢癌细胞系中的铂耐药性。
DOI：
10.1186/1756-9966-30-91
发表时间：
2011-10-04
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Eckstein N
通讯作者：
Eckstein N
影响因子：
5.7
作者：
Chao, Jui-I;Su, Wen-Chi;Liu, Huei-Fang
通讯作者：
Liu, Huei-Fang
影响因子：
3.8
作者：
Bosetti, Cristina;Rossi, Marta;LaVecchia, Carlo
通讯作者：
LaVecchia, Carlo
影响因子：
3.2
作者：
Ling, Yun;Chen, Yan;Guo, Qinglong
通讯作者：
Guo, Qinglong
影响因子：
2.9
作者：
Bosetti, Cristina;Bravi, Francesca;La Vecchia, Carlo
通讯作者：
La Vecchia, Carlo