ROS regulated reversible protein phase separation synchronizes plant flowering
ROS regulated reversible protein phase separation synchronizes plant flowering
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ROS调节可逆蛋白质相分离同步植物开花
DOI：
10.1038/s41589-021-00739-0
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发表时间：
2021-02-25
影响因子：
14.8
通讯作者：
Xu, Cao
中科院分区：
文献类型：
--
作者：
Huang, Xiaozhen;Chen, Shudong;Xu, Cao
好氧生物如何利用不可避免地产生但具有潜在危险的活性氧（ROS）来有益于正常生活是一个基本的生物学问题。据报道，局部积累的ROS引发干细胞分化。然而，潜在的分子机制尚不清楚。在这里，我们揭示了植物芽顶端分生组织（SAM）中发育产生的H2O2触发了可逆的蛋白质相分离的开花花（ESTA），一个转录因子，时间开花的番茄中的过渡抑制SAM的成熟前。半胱氨酸残基在细胞氧化还原过程中形成二硫键，连接多个半胱氨酸分子，并增加内在无序区域的数量，以驱动相分离。氧化触发相分离使拟南芥能够结合并隔离花识别基因ANANTHA的启动子，从而抑制其表达。通过氧化还原调节相分离的可逆转录缩合赋予好氧生物在处理发育线索时基因控制的灵活性。
How aerobic organisms exploit inevitably generated but potentially dangerous reactive oxygen species (ROS) to benefit normal life is a fundamental biological question. Locally accumulated ROS have been reported to prime stem cell differentiation. However, the underlying molecular mechanism is unclear. Here, we reveal that developmentally produced H2O2in plant shoot apical meristem (SAM) triggers reversible protein phase separation of TERMINATING FLOWER (TMF), a transcription factor that times flowering transition in the tomato by repressing pre-maturation of SAM. Cysteine residues within TMF sense cellular redox to form disulfide bonds that concatenate multiple TMF molecules and elevate the amount of intrinsically disordered regions to drive phase separation. Oxidation triggered phase separation enables TMF to bind and sequester the promoter of a floral identity geneANANTHAto repress its expression. The reversible transcriptional condensation via redox-regulated phase separation endows aerobic organisms with the flexibility of gene control in dealing with developmental cues.