Glycyl-tRNA synthetase of Escherichia coli: immunological homology with phenylalanyl-tRNA synthetase.
Glycyl-tRNA synthetase of Escherichia coli: immunological homology with phenylalanyl-tRNA synthetase.
复制标题
大肠杆菌的甘氨酰-tRNA 合成酶：与苯丙氨酰-tRNA 合成酶的免疫同源性。
DOI：
10.1016/0003-9861(88)90391-8
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发表时间：
1988
影响因子：
3.9
通讯作者：
Weber,BH
中科院分区：
文献类型：
--
作者：
Nagel,GM;Johnson,MS;Rynd,J;Petrella,E;Weber,BH
抗大肠杆菌甘氨酰-tRNA合成酶（GlyRS）抗体与大肠杆菌有广泛的交叉反应。大肠杆菌苯丙氨酰-tRNA合成酶（PheRS）。这些数据表明，这两种酶之间存在结构同源性，这两种酶是大肠杆菌中仅有的两种氨酰tRNA合成酶。大肠杆菌具有α2β 2亚基结构。虽然在从已知基因序列推导的蛋白质序列中仅发现有限的相似性，但GlyRS和PheRS中共同表位的存在增加了这些蛋白质之间相当长的物理和化学相似性列表。此外，针对GlyRS的α-和β-亚基的抗体以相同的相对方式抑制GlyRS和PheRS，表明每种酶中亚基的功能和结构相似。与此相反，GlyRS抗体不与弗罗姆的许多其他氨酰-tRNA合成酶活性交叉反应。大肠杆菌、酵母菌或芽孢杆菌。
Antibodies toEscherichia coliglycyl-tRNA synthetase (GlyRS) cross-react extensively withE. coliphenylalanyl-tRNA synthetase (PheRS). These data indicate that structural homology exists between these two enzymes, the only two aminoacyl-tRNA synthetases inE. colihaving an α2β2subunit structure. Although only limited similarities are found in the protein sequences deduced from their known gene sequences, the presence of common epitopes in GlyRS and PheRS adds to a rather long list of physical and chemical similarities between those proteins. In addition, antibodies directed at the α- and β-subunits of GlyRS inhibit both GlyRS and PheRS in the same relative manner, indicating that the function as well as the structure of subunits is similar in each enzyme. In contrast, GlyRS antibodies did not cross-react with a number of other aminoacyl-tRNA synthetase activities fromE. coli, yeast, orBacillus.