Assessment of Real-Time Polymerase Chain Reaction Detection of Acanthamoeba and Prognosis Determinants of Acanthamoeba Keratitis
Assessment of Real-Time Polymerase Chain Reaction Detection of Acanthamoeba and Prognosis Determinants of Acanthamoeba Keratitis
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DOI：
10.1016/j.ophtha.2011.12.023
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发表时间：
2012-06-01
期刊：
影响因子：
13.7
通讯作者：
Yagita, Kenji
中科院分区：
文献类型：
--
作者：
Ikeda, Yoshifumi;Miyazaki, Dai;Yagita, Kenji
目的：目的评价实时荧光聚合酶链反应（PCR）检测阿米巴角膜炎（AK）的诊断价值。此外，以确定术前水肿的决定因素，在眼睛与AK.Design：回顾性，横断面study.Participants：共103例患者的104只眼睛被诊断为AK或细菌或细菌相关性角膜炎（BK）by conventional tests.Methods：29眼AK和75眼BK进行了评估阿米巴和细菌DNA的实时PCR。对AK患者的阿米巴拷贝数、细菌负荷和临床参数进行评估，以确定与不良结局显著相关的因素，即最终视力，
Objective: To evaluate the diagnostic value of real-time polymerase chain reaction (PCR) for detecting Acanthamoeba in eyes diagnosed with Acanthamoeba keratitis (AK) by conventional tests. In addition, to determine the preoperative prognosis-determining factors in eyes with AK.Design: Retrospective, cross-sectional study.Participants: A total of 104 eyes of 103 patients who were diagnosed with AK or with bacterial or bacteria-associated keratitis (BK) by conventional tests.Methods: Twenty-nine eyes with AK and 75 eyes with BK were evaluated for Acanthamoeba and bacterial DNA by real-time PCR. The Acanthamoeba copy numbers, bacterial load, and clinical parameters in the patients with AK were assessed for those significantly associated with poor outcome, that is, final visual acuity of