Use of an Endothelial Monolayer on a Vascular Graft Prior to Implantation: Temporal Dynamics and Compatibility with the Operating Room
Use of an Endothelial Monolayer on a Vascular Graft Prior to Implantation: Temporal Dynamics and Compatibility with the Operating Room
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植入前在血管移植物上使用内皮单层：时间动力学和与手术室的兼容性
DOI：
10.1097/00000658-198606000-00012
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发表时间：
1986
影响因子：
9
通讯作者：
F. Rosato
中科院分区：
文献类型：
--
作者：
B. Jarrell;S. Williams;L. Solomon;L. Speicher;E. Koolpe;J. Radomski;R. Carabasi;D. Greener;F. Rosato
从内皮细胞(ECs)到涤纶(Dacron)的初始接触直到单层的建立，对事件的时间序列进行了研究。将培养的成人内皮细胞进行放射性标记，接种到涤纶上，并在强力洗涤后进行粘附量测定。70%的种植内皮细胞在未经处理的涤纶上2小时，在间质I/III型胶原和羊膜衍生基底膜(IV)胶原表面处理的涤纶上黏附30分钟，在血浆包被的涤纶上黏附10分钟。用扫描电子显微镜(SEM)对平行样品进行形态观察，以评价ECs在表面的粘附性。种植在普通涤纶上的ECS表现出有限的粘附性，而等离子处理的涤纶上的细胞表现出有限的细胞-细胞结合。在基底膜处理的涤纶上，30min时内皮细胞呈现扁平变薄的形态，完全覆盖移植物表面。这一时间框架与大多数血管手术相兼容，使立即内皮化移植成为可能。
The temporal sequence of events was examined from initial contact of endothelial cells (ECs) to Dacron until the establishment of a monolayer. Cultured human adult ECs were radiolabeled, seeded onto Dacron, and adherence was quantified after vigorous washing. Firm adherence of 70% of the seeded ECs was seen by 2 hours to untreated Dacron, by 30 minutes to Dacron pretreated with a combination of interstitial type I/III collagen and an amnion-derived basement membrane (Type IV) collagen surface, and by 10 minutes to plasma-coated Dacron. Parallel samples were examined morphologically by scanning electron microscopy (SEM) to evaluate the adherence of ECs to surfaces. ECs seeded onto plain Dacron exhibited limited adherence, while cells on plasma-treated Dacron exhibited limited cell-cell associations. On basement membrane-treated Dacron, by 30 minutes the ECs exhibited a flat attenuated morphology, completely covering the graft surface. This time-frame is compatible with most vascular procedures, making an immediately endothelialized graft feasible.