Widespread, abundant, and diverse TE-associated siRNAs in developing wheat grain
Widespread, abundant, and diverse TE-associated siRNAs in developing wheat grain
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小麦籽粒发育过程中广泛、丰富且多样的 TE 相关 siRNA
DOI：
10.1016/j.gene.2013.03.101
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发表时间：
2013-06-10
期刊：
影响因子：
3.5
通讯作者：
Yao, Yingyin
中科院分区：
文献类型：
--
作者：
Sun, Fenglong;Guo, Weiwei;Yao, Yingyin
与RNA干扰相关的小RNA是许多发育过程中的关键分子，它们既可以调节发育基因的表达，又可以维持基因组和表观基因组的完整性。在植物中，长度为24 nt的短干扰RNA（siRNA）是与转座因子（TE）、其他重复序列和病毒防御相关的丰富类型的小RNA。TE相关siRNA影响基因组完整性的一种方式是通过称为RNA指导的DNA甲基化（RdDM）的过程改变染色质结构。在本文中，我们描述了一个比较调查的siRNA从小麦幼苗叶片，幼苗根，幼穗，并在授粉后8和15天（DAP）的粮食。我们发现，siRNA分布的一般模式在不同的TE之间和同一家族的TE内是相似的，无论组织如何，但24-nt siRNA的相对丰度在发育中的谷物中是最高的。我们还发现，在胚乳中具有转录活性的TE不仅在谷物中，而且在其他组织中也与最高的siRNA丰度相关。这些结果表明，RdDM是小麦籽粒发育的重要特征，并且与胚乳中TE表达导致TE siRNA增加的假设一致，并且RdDM是植物种子发育的保守特征。皇冠版权所有（C）2013由爱思唯尔B. V.出版保留所有权利。
Small RNAs related to RNA interference are key molecules in many developmental processes, in which they can both regulate developmental gene expression and maintain the integrity of the genome and epigenome. In plants, short interfering RNAs (siRNAs) of 24nt in length are an abundant type of small RNA associated with transposable elements (TEs), other repetitive sequences, and viral defense. One means by which TE-associated siRNAs affect genome integrity is by altering chromatin structure through a process called RNA-directed DNA methylation (RdDM). In this paper, we describe a comparative survey of siRNAs from wheat seedling leaves, seedling roots, young spikelets, and grains at 8 and 15 days after pollination (DAP). We find that the general patterns of siRNA distributions are similar across different TEs and within TEs of the same family regardless of tissue, but the relative abundance of 24-nt siRNAs is highest in developing grains. We also find that TEs that are transcriptionally active in endosperm are associated with the highest siRNA abundance not only in grains, but also in other tissues as well. These results suggest that RdDM is an important feature of developing wheat grain and are consistent with the hypothesis that TE expression in endosperm results in increased TE siRNAs, and that RdDM is a conserved feature of plant seed development. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.