2 MESSENGER-RNAS CAN BE PRODUCED FROM A SINGLE IMMUNOGLOBULIN-MU GENE BY ALTERNATIVE RNA PROCESSING PATHWAYS
2 MESSENGER-RNAS CAN BE PRODUCED FROM A SINGLE IMMUNOGLOBULIN-MU GENE BY ALTERNATIVE RNA PROCESSING PATHWAYS
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DOI：
10.1016/0092-8674(80)90617-0
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
HOOD, L
中科院分区：
文献类型：
--
作者：
EARLY, P;ROGERS, J;HOOD, L
.mu。膜结合的链（.mu.m）和分泌的（.mu.s）形式的Ig [免疫球蛋白] M由2种mRNA编码。克隆的C [互补] DNA从2 .mu产生。 M104E小鼠骨髓瘤肿瘤的mRNA仅在其3'末端有所不同，该末端编码.mu.m或.mu.s c末端。 .mu.m和.mu.s mRNA是由单个.mu的转录本产生的。基因。 .mu.s mRNA的最后187个核苷酸源自与编码C.Mu.4域的序列的3'端的DNA连续。 .mu.m cDNA克隆不包括这187个核苷酸，而是包含来自位于1850 bp 3''c.mu.4序列的2个外显子的392个核苷酸。基因组和cDNA序列的比较表明，在.mu.m mRNA中，1850年核苷酸的RNA剪接在c.mu.4结束时在编码序列中与位点相连，并在第一个膜特异性外显子的开始时与位点相连。 118个核苷酸的第二个RNA剪接与从第一和第二膜特异性外显子转录的序列。 .mu.m和.mu.s cDNA之间观察到的差异表明，将poly（a）添加到.mu的转录本的位点的发育控制。基因确定.mu.m或.mu.s链合成的相对水平。控制.mu的可能模型。讨论了基因转录物以及这种形式的发育调节的RNA处理对真核分裂基因进化的重要性。
The .mu. chains of the membrane-bound (.mu.m) and secreted (.mu.s) forms of Ig[immunoglobulin] M are encoded by 2 species of mRNA. Cloned c[complementary]DNA produced from the 2 .mu. mRNA of M104E mouse myeloma tumors differ only at their 3'' ends, which encode either the .mu.m or .mu.s C terminus. The .mu.m and .mu.s mRNA are produced from transcripts of a single .mu. gene. The last 187 nucleotides of .mu.s mRNA are derived from DNA contiguous with the 3'' end of the sequence encoding the C.mu.4 domain. The .mu.m cDNA clone does not include these 187 nucleotides, but instead contains 392 nucleotides derived from 2 exons located 1850 bp 3'' to the C.mu.4 sequence. Comparison of genomic and cDNA sequences show that in .mu.m mRNA, an RNA splice of 1850 nucleotides joins a site in the coding sequence at the end of C.mu.4 with a site at the beginning of the 1st membrane-specific exon. A 2nd RNA splice of 118 nucleotides joins sequences transcribed from the 1st and 2nd membrane-specific exons. The differences observed between .mu.m and .mu.s cDNA suggest that developmental control of the site at which poly(A) is added to transcripts of the .mu. gene determines the relative levels of .mu.m or .mu.s chain synthesis. Possible models for the control of .mu. gene transcripts and the significance of this form of developmentally regulated RNA processing for the evolution of eukaryotic split genes were discussed.