SPECT imaging of myocardial infarction using 99mTc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model
SPECT imaging of myocardial infarction using 99mTc-labeled C2A domain of synaptotagmin I in a porcine ischemia-reperfusion model
复制标题
在猪缺血再灌注模型中使用突触结合蛋白 I 的 99mTc 标记的 C2A 结构域对心肌梗死进行 SPECT 成像。
DOI：
10.1016/j.nucmedbio.2007.06.014
复制
发表时间：
2007-11-01
影响因子：
3.1
通讯作者：
Zhao, Ming
中科院分区：
文献类型：
--
作者：
Fang, Wei;Wang, Feng;Zhao, Ming
简介：突触结合蛋白I的C2 A结构域通过与暴露的阴离子磷脂结合来识别坏死和凋亡细胞。我们的目标是探索潜在的成像效用的Tc-99 m标记的C2 A在检测急性心肌细胞死亡的猪模型，类似于人类cardiovascular physiology.Methods：缺血（20-25分钟）诱导猪（M/F，20-25公斤）使用球囊血管成形术。再灌注后1-2 h静脉注射Tc-99 m-C2 A-GST（n=7）或Tc-99 m-BSA（n=2）。非梗死动物注射99 mTc-C2 A-GST（n=4）。在注射后3和6 h采集SPECT图像。心肌组织进行了分析，以确认存在的细胞death.Results：局灶性摄取检测到在5个7个科目在3小时，在所有的梗死科目在6小时postinjection，但不是在梗死动物注射Tc-99 m-BSA或在noninfarction动物与Tc-99 m-C2 A-GST。γ计数梗死与正常心肌产生了10.2 +/- 5.7倍的绝对放射性升高，与组织学证实infarct.Conclusions：我们目前的数据在猪心肌梗死急性期成像心肌细胞死亡。C2 A作为一种梗死亲分子探针具有前景并值得进一步开发。(c)2007年爱思唯尔公司All rights reserved.
Introduction: The C2A domain of synaptotagmin I recognizes necrotic and apoptotic cells by binding to exposed anionic phospholipids. The goal is to explore the potential imaging utility of Tc-99m-labeled C2A in the detection of acute cardiac cell death in a porcine model that resembles human cardiovascular physiology.Methods: Ischemia (20-25 min) was induced in pigs (M/F, 20-25 kg) using balloon angioplasty. Tc-99m-C2A-GST (n=7) or Tc-99m-BSA (n=2) was injected intravenously 1-2 h after reperfusion. Noninfarct animals were injected with 99mTc-C2A-GST (n=4). SPECT images were acquired at 3 and 6 h postinjection. Cardiac tissues were analyzed to confirm the presence of cell death.Results: Focal uptake was detected in five out of seven subjects at 3 h and in all infarct subjects at 6 h postinjection but not in infarct animals injected with Tc-99m-BSA or in noninfarct animals with Tc-99m-C2A-GST. Gamma counting of infarct versus normal myocardium yielded a 10.2 +/- 5.7-fold elevation in absolute radioactivity, with histologically confirmed infarction.Conclusions: We present data on imaging myocardial cell death in the acute phase of infarction in pigs. C2A holds promise and warrants further development as an infarct-avid molecular probe. (c) 2007 Elsevier Inc. All rights reserved.