RETROVIRUS INSERTION INTO HERPESVIRUS IN VITRO AND IN VIVO
RETROVIRUS INSERTION INTO HERPESVIRUS IN VITRO AND IN VIVO
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DOI：
10.1073/pnas.89.3.991
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发表时间：
1992-02-01
影响因子：
11.1
通讯作者：
KUNG, HJ
中科院分区：
文献类型：
--
作者：
ISFORT, R;JONES, D;KUNG, HJ
逆转录病毒和疱疹病毒是自然发生的人和动物的病原体。同一宿主同时感染这两种病毒是很常见的。我们在这里报道，逆转录病毒可以直接整合到疱疹病毒基因组中。具体地说，我们展示了一种非急性逆转录病毒，网状内皮组织增生症病毒(REV)，插入到疱疹病毒，马立克病病毒(MDV)中。这两种病毒都能诱发鸡的T淋巴瘤，而且通常在同一动物体内共存。REV DNA整合到MDV中发生在最近减毒的MDV毒株和短期的体外联合感染实验中。我们还提供了REV过去曾插入MDV致病毒株的提示性证据。与REV长末端重复序列同源的序列在致癌MDV中被发现，但在非致癌毒株中不被发现。这些结果增加了逆转录病毒信息可能通过疱疹病毒传播的可能性，以及逆转录病毒元件可以调节疱疹病毒的表达。此外，逆转录病毒可能通过插入突变提供一个有用的工具来表征疱疹病毒的功能。
Retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. Coinfection of the same host with both these viruses is common. We report here that a retrovirus can integrate directly into a herpesvirus genome. Specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (REV), into a herpesvirus, Marek disease virus (MDV). Both viruses are capable of inducing T lymphomas in chickens and often coexist in the same animal. REV DNA integration into MDV occurred in a recently attenuated strain of MDV and in a short-term coinfection experiment in vitro. We also provide suggestive evidence that REV has inserted into pathogenic strains of MDV in the past. Sequences homologous to the REV long terminal repeat are found in oncogenic MDV but not in nononcogenic strains. These results raise the possibility that retroviral information may be transmitted by herpesvirus and that herpesvirus expression can be modulated by retroviral elements. In addition, retrovirus may provide a useful tool to characterize herpesviral function by insertional mutagenesis.