A CHONDROGENIC CELL-LINE DERIVED FROM A DIFFERENTIATING CULTURE OF AT805 TERATOCARCINOMA CELLS
A CHONDROGENIC CELL-LINE DERIVED FROM A DIFFERENTIATING CULTURE OF AT805 TERATOCARCINOMA CELLS
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DOI：
10.1016/0922-3371(90)90079-c
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发表时间：
1990-05-01
期刊：
影响因子：
--
通讯作者：
IKAWA, Y
中科院分区：
文献类型：
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作者：
ATSUMI, T;MIWA, Y;IKAWA, Y
从AT 805畸胎癌的分化培养物中分离的细胞系ATDC 5在生长期表达成纤维细胞表型。向培养基中加入10 μ g/ml胰岛素，细胞甚至在融合后阶段继续生长，形成软骨结节样细胞聚集体，用阿尔新蓝染色并产生软骨特异性蛋白聚糖和II型胶原，它们是软骨形成的典型标记分子。由于ATDC 5细胞也分化成不可识别的色素细胞，因此它们显然由未确定的细胞组成。因此，ATDC 5提供了一个很好的模型系统，了解软骨分化。
A cell line, ATDC5, isolated from a differentiating culture of AT805 teratocarcinoma expressed a fibroblastic cell phenotype in a growing phase. With the addition of 10 .mu.g/ml insulin to the medium, cells continued to grow even in a postconfluent phase, formed cartilage nodule-like cell aggregates, were stained with Alcian blue and produced cartilage-specific proteoglycan and type II collagen, typical marker molecules for chondrogenesis. Since ATDC5 cells also differentiated into unidentifiable pigmented cells, they are apparently composed of undetermined cells. ATDC5, therefore, provides a good model system with which to understand chondrogenic differentiation.