Effect of growth phase and cell envelope structure on susceptibility of Salmonella typhimurium to the lactoperoxidase-thiocyanate-hydrogen peroxide system
Effect of growth phase and cell envelope structure on susceptibility of Salmonella typhimurium to the lactoperoxidase-thiocyanate-hydrogen peroxide system
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生长期和细胞膜结构对鼠伤寒沙门氏菌对乳过氧化物酶-硫氰酸盐-过氧化氢系统敏感性的影响
DOI：
10.1128/iai.39.3.1187-1195.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
W. E. White
中科院分区：
文献类型：
--
作者：
M. Purdy;J. Tenovuo;K. Pruitt;W. E. White
发现乳过氧化物酶-硫氰酸盐-过氧化氢系统对鼠伤寒沙门氏菌菌株具有抑菌和杀菌活性。杀菌活性明显依赖于细菌细胞包膜的渗透性。具有最具渗透性的细胞包膜的深粗糙突变体TA 1535在中性和酸性pH下都被杀死，而在亲本菌株hisG 46的完整细胞中观察到很少或没有杀死。δ半乳糖突变体，TA 1530，代表一个中间的细胞被膜渗透性，被抑制的程度比TA 1535小得多。对数生长期的细菌比稳定期的细菌对杀菌效果更敏感。生长阶段对抑菌效果影响不大。hisG 46菌株在葡萄糖存在下产生显著量的酸。乳酸过氧化物酶-硫氰酸盐-过氧化氢系统抑制了这种酸的产生，并且与用几种链球菌菌株获得的结果相反，这种抑制通过加入还原剂（2-巯基乙醇）而不被逆转。
The lactoperoxidase-thiocyanate-hydrogen peroxide system was found to have both bacteriostatic and bactericidal activities against strains of Salmonella typhimurium. The bactericidal activity was clearly dependent on the permeability of the bacterial cell envelope. The deep rough mutant TA1535, with the most permeable cell envelope, was killed both at neutral and acid pH, whereas very little or no killing was observed with the intact cells of the parent strain hisG46. The delta gal mutant, TA1530, representing an intermediate in cell envelope permeability, was inhibited to a much lesser extent than TA1535. Bacteria in log phase of growth were more sensitive to the bactericidal effects than were those in stationary phase. Growth phase had little influence on the bacteriostatic effects. The hisG46 strain produced significant quantities of acid in the presence of glucose. This acid production was inhibited by the lactoperoxidase-thiocyanate-hydrogen peroxide system, and, in contrast to results obtained with several strains of streptococci, this inhibition was not reversed by addition of a reducing agent (2-mercaptoethanol).
乳过氧化物酶催化的硫氰酸盐氧化：硫氰酸盐氧化形式之间的平衡。
影响因子：
2.9
作者：
Thomas,EL
通讯作者：
Thomas,EL
人乳过氧化物酶源自乳汁白细胞。
DOI：
10.1016/0304-4165(82)90014-9
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Moldoveanu,Z;Tenovuo,J;Mestecky,J;Pruitt,KM
通讯作者：
Pruitt,KM
特异性免疫因子和先天免疫因子的相互作用：IgA 增强乳过氧化物酶系统对变形链球菌的抗菌作用。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Tenovuo,J;Moldoveanu,Z;Mestecky,J;Pruitt,KM;Rahemtulla,BM
通讯作者：
Rahemtulla,BM