Effects of serum from patients with type 1 diabetes on primary cerebellar granule cells
Effects of serum from patients with type 1 diabetes on primary cerebellar granule cells
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DOI：
10.2337/diabetes.50.2007.s77
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发表时间：
2001-02-01
期刊：
影响因子：
7.7
通讯作者：
Orrenius, S
中科院分区：
文献类型：
--
作者：
Chandra, J;Yang, SN;Orrenius, S
1型糖尿病是一种病因不明的自身免疫性疾病。我们以前的工作表明，1型糖尿病患者血清中存在的一种因子会导致胰岛β细胞钙通道活性增加和DNA片段化。在这里，我们研究了1型糖尿病血清对原代小脑颗粒细胞(CGCs)的影响。在CGCs中，暴露于1型糖尿病血清没有引起细胞凋亡增加或钙通道活性改变。然而，患者血清确实在一种具有三角形胞体的细胞类型中引起了钙信号的调制，这种细胞表现出低电压门控的钙电流。这种细胞主要存在于接触1型糖尿病血清的培养物中。低电压门控钙电流和长的神经元树突的存在表明这种独特的细胞是神经元起源的，而不是神经胶质起源的。
Type 1 diabetes is an autoimmune disease of unknown etiology. Our previous work has shown that a factor present in serum from type 1 diabetic patients causes increased Ca2+ channel activity and apoptotic DNA fragmentation in pancreatic beta -cells. Here we examined the effects of type 1 diabetic serum on primary cerebellar granule cells (CGCs). In CGCs, exposure to type 1 diabetic serum did not cause increased apoptosis or changes in Ca2+ channel activity. However, patient serum did cause modulation of Ca2+ signals in a cell type with triangular soma that exhibited low voltage-gated Ca2+ currents. This cell was present primarily in cultures exposed to type 1 diabetic serum. The presence of low voltage-gated Ca2+ currents and long neuronal dendrites indicated that this unique cell was of neuronal origin and not of glial origin.