Soft-state biomicrofluidic pulse generator for single cell analysis
Soft-state biomicrofluidic pulse generator for single cell analysis
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DOI：
10.1063/1.2195106
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发表时间：
2006-05-01
影响因子：
4
通讯作者：
Lee, LP
中科院分区：
文献类型：
--
作者：
Sabounchi, P;Ionescu-Zanetti, C;Lee, LP
我们提出了一种用于单细胞分析的软态生物微流控脉冲发生器的设计、制造和表征。通过横向微流体连接的流体动力学细胞捕获允许从大量悬浮液中捕获单个细胞。邻近细胞捕获通道的微流体注射位点能够脉冲输送纳升体积的生化试剂。我们展示了在10 Hz的频率下的试剂的应用和去除，上升时间小于33 ms，试剂消耗速率为0.2 nL/s。它示出，该系统作为一个低通滤波器与截止频率为7 Hz。
We present the design, fabrication, and characterization of a soft-state biomicrofluidic pulse generator for single cell analysis. Hydrodynamic cell trapping via lateral microfluidic junctions allows the trapping of single cells from a bulk suspension. Microfluidic injection sites adjacent to the cell-trapping channels enable the pulsed delivery of nanoliter volumes of biochemical reagent. We demonstrated the application and removal of reagent at a frequency of 10 Hz with a rise time of less than 33 ms and a reagent consumption rate of 0.2 nL/s. It is shown that this system operates as a low-pass filter with a cutoff frequency of 7 Hz.