Sequence analyses of the ITS regions and the matK gene for determining phylogenetic relationships of Diospyros kaki (persimmon) with other wild Diospyros (Ebenaceae) species
Sequence analyses of the ITS regions and the matK gene for determining phylogenetic relationships of Diospyros kaki (persimmon) with other wild Diospyros (Ebenaceae) species
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DOI：
10.1007/s11295-007-0096-y
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发表时间：
2008-04
影响因子：
2.4
通讯作者：
K. Yonemori;C. Honsho;S. Kanzaki;H. Ino;A. Ikegami;A. Kitajima;A. Sugiura;D. Parfitt
中科院分区：
文献类型：
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作者：
K. Yonemori;C. Honsho;S. Kanzaki;H. Ino;A. Ikegami;A. Kitajima;A. Sugiura;D. Parfitt
为了阐明柿与以往研究中亲缘关系密切的物种之间的亲缘关系，对其核糖体内转录间隔区(ITS)DNA序列和叶绿体蛋白K基因进行了测序，并与9种泰国柿、4种温带柿和1种南部非洲柿进行了比较。最大简约性、最大似然性和邻接性分析表明，TEMK及其数据集。Kakias与油茶和油茶这两个二倍体物种关系密切。腺体、腺体和腺体的分布。油菜在ITS和MatK数据集中的位置不同，这表明在这些物种的发育过程中可能发生了杂交和/或导入。产于泰国的二倍体种Ehretoides。这些结果不同于先前用3.2-和2.1-kb扩增序列中的叶绿体DNA(CpDNA)限制位点突变对该属进行的分析。这一结果支持了Ng的假说。腺苷和D。Kakima可能有一个共同的祖先，D oleifera也与D密切相关。卡基。
To elucidate the relationships amongDiospyros kakiand species closely related in previous studies, the nuclear ribosomal internal transcribed spacer (ITS) DNA sequence and the chloroplastmatK gene were sequenced and compared with those of nineDiospyrosspecies from Thailand, four species from temperate regions, and one species of southern Africa,D. lycioides. Maximum parsimony, maximum likelihood, and neighbor joining analyses of thematK and ITS data sets revealed thatD. kakiis closely related to two diploid species,D. oleiferaandD. glandulosa.D. kaki,D. glandulosa, andD. oleiferawere placed differently in the trees obtained from ITS andmatK data sets, suggesting that hybridization and/or introgression may have occurred during the development of these species.D. kakiwas not found to be closely related toD. ehretioides, a diploid species from Thailand. These results differed from a prior analysis of this genus performed with chloroplast DNA (cpDNA) restriction site mutations in 3.2- and 2.1-kb amplified sequences. The results supported Ng’s hypothesis thatD. glandulosaandD. kakimay share a common ancestor.D. oleiferawas also closely associated withD. kaki.