Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats.
Plasma Exosomal miRNA-122-5p and miR-300-3p as Potential Markers for Transient Ischaemic Attack in Rats.
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血浆外泌体 miRNA-122-5p 和 miR-300-3p 作为大鼠短暂性脑缺血发作的潜在标志物
DOI：
10.3389/fnagi.2018.00024
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发表时间：
2018
影响因子：
4.8
通讯作者：
Qin C
中科院分区：
文献类型：
--
作者：
Li DB;Liu JL;Wang W;Luo XM;Zhou X;Li JP;Cao XL;Long XH;Chen JG;Qin C
背景：在溶栓时间窗内鉴别短暂性脑缺血发作（TIA）和缺血性卒中是困难的。虽然TIA可以在此窗口内诊断，但最新的成像技术复杂且昂贵。血清标志物具有无创、快速、经济等优点，可用于多种疾病的诊断和预后判断。TIA的外泌体来源的miRNA标志物是未知的。方法：我们检查了大鼠大脑中动脉（MCAo）阻塞5 min、10 min和2 h造成的局灶性脑缺血。通过深度测序和定量实时聚合酶链反应（qRT-PCR）鉴定了脑脊液（CSF）和血浆中具有一致趋势的外泌体miRNA。使用受试者工作特征（ROC）曲线的曲线下面积（AUC）来评估这些miRNA对大鼠TIA的诊断准确性。结果：筛选出rno-miR-122- 5 p和rno-miR-300- 3 p。与对照和5分钟血浆相比，在10分钟缺血大鼠中血浆外泌体rno-miR-122- 5 p显著下调。与对照、10 min和2 h大鼠相比，5 min缺血大鼠血浆外泌体rno-miR-300- 3 p显著上调。这些miRNA的血浆和CSF水平相关。ROC分析显示，与对照相比，10和5 min大鼠中rno-miR-122- 5 p（0.960）和rno-miR-300- 3 p（0.970）的AUC值分别较高。结论：血浆外泌体rno-miR-122- 5 p和rno-miR-300- 3 p可能是TIA的血液生物学标志物。
Background: Differentiation of transient ischaemic attack (TIA) from ischaemic stroke within the thrombolysis time window is difficult. Although TIA may be diagnosed within this window, the latest imaging technologies are complex and costly. Serum markers, which are non-invasive, rapid and economic, are used for diagnosis and prognosis of various diseases. Exosome-derived miRNA markers for TIA are unknown. Methods: We examined focal brain ischaemia produced by occlusion of the middle cerebral artery (MCAo) for 5 min, 10 min, and 2 h in rats. Exosomal miRNAs with consistent trends in cerebrospinal fluid (CSF) and plasma were identified by deep sequencing and quantitative real-time polymerase chain reaction (qRT-PCR). The areas under the curve (AUC) of the receiver operating characteristic (ROC) curve were used to evaluate the diagnostic accuracy of these miRNAs for TIA in rats. Results: Rno-miR-122-5p and rno-miR-300-3p were selected. Plasma exosomal rno-miR-122-5p was significantly downregulated in 10 min ischaemic rats compared with control and 5 min plasma. Plasma exosomal rno-miR-300-3p was significantly upregulated in 5 min ischaemic rats compared with control, 10 min and 2 h rats. Plasma and CSF levels of these miRNAs were correlated. ROC analysis showed high AUC values for rno-miR-122-5p (0.960) and rno-miR-300-3p (0.970) in the 10 and 5 min rats, respectively, compared with controls. Conclusions: Plasma exosomal rno-miR-122-5p and rno-miR-300-3p may be blood-based TIA biomarkers.
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影响因子：
6
作者：
Bakker, FC;Klijn, CJM;Kappelle, LJ
通讯作者：
Kappelle, LJ
影响因子：
20.3
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Njock, Makon-Sebastien;Cheng, Henry S.;Fish, Jason E.
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Wang, Pei-Chang
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3.7
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Hill AF
影响因子：
11.1
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Balusu S;Van Wonterghem E;De Rycke R;Raemdonck K;Stremersch S;Gevaert K;Brkic M;Demeestere D;Vanhooren V;Hendrix A;Libert C;Vandenbroucke RE
通讯作者：
Vandenbroucke RE