Proteome dynamics during post-desiccation recovery reveal convergence of desiccation and gamma radiation stress response pathways in Deinococcus radiodurans.
Proteome dynamics during post-desiccation recovery reveal convergence of desiccation and gamma radiation stress response pathways in Deinococcus radiodurans.
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DOI：
10.1016/j.bbapap.2017.06.014
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发表时间：
2017-09
期刊：
影响因子：
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通讯作者：
A. K. Ujaoney;M. Padwal;Bhakti Basu
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文献类型：
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作者：
A. K. Ujaoney;M. Padwal;Bhakti Basu
耐辐射球菌天生对电离辐射和干燥都有抵抗力。15个月的干燥被发现是ld50剂量的forD。耐辐射奇球菌。d .干燥细胞。在干燥后恢复（PDR）期间，放射性废物进入了6小时的生长停滞。通过二维凝胶电泳结合质谱法解析细胞蛋白，绘制了PDR期间的蛋白质组动力学。在整个PDR中，至少有41种蛋白质（由蛋白质组谱上的51个点表示）存在差异表达。参与单链退火的DNA修复蛋白（DdrA和DdrB）、核苷酸切除修复蛋白（UvrA和UvrB）、同源重组蛋白（RecA）以及其他参与DNA复制、重组和修复的重要蛋白（Ssb、GyrA和GyrB）的表达均大幅上调。CRP/FNR家族转录调节因子（CRP）在整个PDR期间保持高表达。其他途径，如细胞解毒、蛋白质稳态和代谢均显示适度诱导和抑制蛋白质。详细讨论了蛋白质组学调节与生存干燥胁迫的功能相关性。将我们的数据与已发表的文献进行比较，揭示了d的辐射和干燥应力反应的收敛性。耐辐射奇球菌。这是第一个证实了d抗辐射胁迫假说的报告。耐辐射性与其抗干燥应力有关。
Deinococcus radiodurans is inherently resistant to both ionizing radiation and desiccation. Fifteen months of desiccation was found to be the LD50dose forD. radiodurans. Desiccated cells ofD. radioduransentered 6 h of growth arrest during post-desiccation recovery (PDR). Proteome dynamics during PDR were mapped by resolving cellular proteins by 2-dimensional gel electrophoresis coupled with mass spectrometry. At least 41 proteins, represented by 51 spots on proteome profiles, were differentially expressed throughout PDR. High upregulation in expression was observed for DNA repair proteins involved in single strand annealing (DdrA and DdrB), nucleotide excision repair (UvrA and UvrB), homologous recombination (RecA) and other vital proteins that contribute to DNA replication, recombination and repair (Ssb, GyrA and GyrB). Expression of CRP/FNR family transcriptional regulator (Crp) remained high throughout PDR. Other pathways such as cellular detoxification, protein homeostasis and metabolism displayed both, moderately induced and repressed proteins. Functional relevance of proteomic modulations to surviving desiccation stress is discussed in detail. Comparison of our data with the published literature revealed convergence of radiation and desiccation stress responses ofD. radiodurans. This is the first report that substantiates the hypothesis that the radiation stress resistance ofD. radioduransis incidental to its desiccation stress resistance.