Nanoprinting onto cells
Nanoprinting onto cells
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DOI：
10.1098/rsif.2005.0104
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发表时间：
2006-06-22
影响因子：
3.9
通讯作者：
Gadegaard, Nikolaj
中科院分区：
文献类型：
--
作者：
Curtis, Adam S. G.;Dalby, Matthew J.;Gadegaard, Nikolaj
在具有纳米形貌信号的表面上生长细胞使细胞基因表达和下游表型变化发生许多变化，但到目前为止，其机制尚不清楚。我们考虑的问题是，地形是否直接nanoimprint到细胞作为一个组成部分的信号转导系统。我们从SEM，TEM和荧光检测的细胞骨架成分的安排提出的证据是一致的，细胞是由基板纳米压印的可能性。纳米打印不干扰整合素介导的粘附过程，并且可能通过它们起作用。细胞从纳米形貌区域移动到。AT区域和反之亦然表明纳米压印花费16小时出现在细胞上，并且当细胞从A移动时花费类似的时间消失。从表面到纳米地形再到背面这种细胞的纳米打印似乎是一种新型的细胞信号传导。
Growing cells on surfaces bearing nanotopography signals makes many changes in cell gene expression and downstream changes in phenotype but the mechanisms for this have, so far, been obscure. We consider the question of whether the topography directly nanoimprints onto the cell as a component of the signal transduction system. Evidence we present from SEM, TEM and fluorescence detection of the arrangements of cytoskeletal components is consistent with the possibility that cells are nanoimprinted by the substrate. The nanoprinting does not interfere with integrin-mediated adhesion processes and may perhaps work through them. Time-lapse video studies of cells moving from areas bearing nanotopography to. at areas and vice versa suggests that the nanoimprinting takes 1 6 h to appear on the cell and a similar time to disappear when the cell moves from a. at surface to a nanotopographic one and back. This nanoprinting of cells would appear to be a novel type of cell signalling.