Acid phosphatase localization in normal and dystrophic retinal pigment epithelium.
Acid phosphatase localization in normal and dystrophic retinal pigment epithelium.
复制标题
酸性磷酸酶定位于正常和营养不良的视网膜色素上皮。
DOI：
10.1007/bf01148115
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
McLaughlin,BJ
中科院分区：
文献类型：
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作者：
Seyfried-Williams,R;McLaughlin,BJ
在这项研究中，酸性磷酸酶（ACPase）定位于正常和皇家外科医学院（RCS）大鼠的视网膜色素上皮（RPE），粉红色的眼睛和遗传性视网膜营养不良的色素，以确定两种底物，Na-β-甘油磷酸和胞苷-5 ' -单磷酸在吞噬后阶段的染色差异。在正常RPE中，高尔基体、溶酶体和吞噬体均呈acpase阳性。在吞噬光感受器杆外节的能力减弱的营养不良型RPE中，ACPase染色定位于色素营养不良型RPE的黑素体和粉红色眼营养不良型RPE的顶端微绒毛膜上，但在粉红色眼和色素营养不良型RPE的吞噬体上均未发现ACPase染色。由于在体内营养不良的RPE中，在任何给定的时间内只能看到很少的吞噬体，因此我们使用组织扩增系统来检测正常和RCS RPE吞噬的乳胶珠的数量，以及正常和突变RPE中同时含有乳胶珠和ACPase活性的吞噬体的数量。我们的研究结果表明，在营养不良中，acpase阳性的吞噬体比正常的少，并且一些酶可能不适当地分流到顶端微绒毛或黑素体而不是吞噬溶酶体。
In this study acid phosphatase (ACPase) was localized in the retinal pigment epithelium (RPE) of normal and Royal College of Surgeons (RCS) rats pink-eyed and pigmented with inherited retinal dystrophy to determine differences in staining during the post-engulfment stages of phagocytosis using two substrates, Na-β-glycerophosphate and cytidine-5′-monophosphate. Staining was similar using either substrate and in the normal RPE the Golgi system, lysosomes and phagosomes were ACPase-positive. In the dystrophic RPE, which has a diminished capacity to phagocytose photoreceptor rod outer segments, ACPase staining was localized on melanosomes in the pigmented dystrophic and on the apical microvillous membranes in the pink-eyed dystrophic, but was not localized on phagosomes in either the pink-eyed or pigmented dystrophic RPE. Since only a few phagosomes were seen at any given time in dystrophic RPE in vivo, a tissue expiant system was used to examine the number of latex beads phagocytosed by normal and RCS RPE, as well as the number of phagosomes containing both beads and ACPase activity in the normal and mutant RPE. Our findings indicate that in the dystrophic, fewer phagosomes are ACPase-positive than in the normal, and that some enzyme may be inappropriately shunted to either the apical microvilli or to melanosomes instead of to phagolysosomes.